, Volume 59, Issue 1, pp 11–16 | Cite as

Formation of embryoid bodies from mouse embryonic stem cells cultured on silicon-coated surfaces

  • Fardin FathiEmail author
  • Taki Altiraihi
  • Seyed Javad Mowla
  • Mansoreh Movahedin
Technical Note


Embryoid bodies (EBs) are primitive embryonic structures derived from differentiating embryonic stem cells (ESCs). Many techniques have been used to obtain EBs. Improving the technique of EB formation can help in achieving better results in ESCs differentiation into neurons, myocardiocytes, haemopoeitic cells, and others. We evaluated the use of Sigmacote™ as a hydrophobic substrate to improve EB formation. CCE and P19 cell lines were used to obtain EBs and retinoic acid was used to induce neural differentiation. The results revealed that Sigmacote™, as a hydrophobic substrate, can improve EB formation from ESCs. Our results demonstrate that the silicon-coating of glass petri dishes by Sigmacote™ is an easy and reproducible technique to enhance EB formation from murine ESCs and EC cells.


Embryoid body Hydrophobic substrate Sigmacote P19 cell line CCE cell line 


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Fardin Fathi
    • 1
    Email author
  • Taki Altiraihi
    • 2
  • Seyed Javad Mowla
    • 3
  • Mansoreh Movahedin
    • 2
  1. 1.Department of Anatomy, KDRC, Faculty of MedicineKurdistan University of Medical SciencesSanandajIran
  2. 2.Department of Anatomy, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran
  3. 3.Department of Genetics, Faculty of Basic SciencesTarbiat Modares UniversityTehranIran

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