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Monitoring pH and dissolved oxygen in mammalian cell culture using optical sensors

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Abstract

Here, we have studied two parameters critical to process control in mammalian cell culture; dissolved oxygen (dO2) and pH, measured with fluorescent sensors thus allowing the study of the metabolic state of cells in culture without removing or damaging cells during cultivation. Two cell lines, namely, NS0 and CHO were batch-grown in 24-well plates at different serum concentrations with the sensors implemented in the bottom of each well. The data showed a good relationship between the dO2 and pH data obtained from fluorescent probes and the growth and death characteristics of cells. The method has provided a high throughput on-line multi-parametric analysis of mammalian cell cellular activity.

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Acknowledgment

This work is funded by a Science Foundation Ireland PI award.

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Authors and Affiliations

  1. School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Dublin, 4, Ireland

    Mariam Naciri, Darrin Kuystermans & Mohamed Al-Rubeai

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  1. Mariam Naciri
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  2. Darrin Kuystermans
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  3. Mohamed Al-Rubeai
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Correspondence to Mariam Naciri.

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Naciri, M., Kuystermans, D. & Al-Rubeai, M. Monitoring pH and dissolved oxygen in mammalian cell culture using optical sensors. Cytotechnology 57, 245–250 (2008). https://doi.org/10.1007/s10616-008-9160-1

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  • DOI: https://doi.org/10.1007/s10616-008-9160-1

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