Abstract
We developed a substitute for serum to produce fed-batch cultures of hybridoma cells in serum-free medium and confirmed that the cells could be successfully cultivated this way. Our substitute consisted of 12 components. The specific production rates of lactate and ammonia, which are harmful byproducts from the cells, were significantly reduced compared with a conventional serum-containing batch culture. This reduction led to a higher cell concentration and a longer production lifetime. As a result, the final concentration of monoclonal antibody was 400 mg/L, or five times greater than that in the conventional serum-containing batch culture. The developed substitute is expected to enable fed-batch cultivation in a serum-free condition.
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Abbreviations
- ΔVF :
-
Volume of feeding medium fed to the reactor since the nth sample was taken, L
- β:
-
Total stoichiometric coefficient of glucose, amino acids, and vitamins, nmole cell−1
- Nt :
-
Total cell number in the reactor at culture time t, number of cells
- Nt,n :
-
Total cells in the reactor when the nth sample was taken, number of cells
- Ct :
-
Total concentration of glucose, amino acids, and vitamins in the supplemental medium, mM
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Shibuya, K., Haga, R. & Namba, M. A serum substitute for fed-batch culturing of hybridoma cells. Cytotechnology 57, 187–197 (2008). https://doi.org/10.1007/s10616-008-9155-y
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DOI: https://doi.org/10.1007/s10616-008-9155-y