Development of primary cell culture from Scylla serrata
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This paper reports for the first time, the Primary cell culture of hepatopancreas from edible crab Scylla serrata using crab saline, L-15 (Leibovitz), 1 × L-15 + crab saline, 2 × L-15 + crab saline, 3 × L-15 and citrate buffer without any serum. We could isolate and maintain E (Embryonalzellen), F (Fibrenzellen), B (Blasenzellen), R (Restzellen) and G (Granular cells). Upon seeding the hepatopancreatic E, F, B, and R cells showed different survival pattern over time than granular cells. A modified L-15 (3×) medium supported the best survival of hepatopancreatic E, F B, and R cells in in-vitro culture. However granular cells could be maintained for 184 days with L-15 (1×) + crab saline. Fetal bovine serum was not effective additive and hampered cell viability in present study.
KeywordsHepatopancreas Scylla serrata Primary cultures E (Embryonalzellen) F (Fibrenzellen) B (Blasenzellen) R (Restzellen) and G (Granular cells)
The authors acknowledge the receipts of financial assistances from Department of Biotechnology, New Delhi under the project number BT/PR8029/AAQ/03/287/2006.
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