Expansion of human hematopoietic stem cells for transplantation: trends and perspectives
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Umbilical cord blood transplantation is clinically limited by its low progenitor cell content. Ex vivo expansion has become an alternative to increase the cell dose available for transplants. Expansion has been evaluated in several ways such as static cultures combining growth factors or mimicking the natural microenvironment using co-culture systems. However, static cultures have a small volume capacity and therefore large-scale expansion has been addressed using bioreactors. These and other biotechnological approaches for the expansion of hematopoietic progenitors and their utility to study several aspects of hematopoietic stem cell biology are discussed here.
Keywords2D-culture 3D-culture Human cells Leukemia Rotating wall vessel Stirred tank Transplant
This work was partially supported by CONACYT-Fondos Sectoriales Salud Grant 13993 and Grant I0010-2007-01, the sabbatical fellowship 79973 and PhD scholarship 103403.
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