Expansion of human hematopoietic stem cells for transplantation: trends and perspectives
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Umbilical cord blood transplantation is clinically limited by its low progenitor cell content. Ex vivo expansion has become an alternative to increase the cell dose available for transplants. Expansion has been evaluated in several ways such as static cultures combining growth factors or mimicking the natural microenvironment using co-culture systems. However, static cultures have a small volume capacity and therefore large-scale expansion has been addressed using bioreactors. These and other biotechnological approaches for the expansion of hematopoietic progenitors and their utility to study several aspects of hematopoietic stem cell biology are discussed here.
Keywords2D-culture 3D-culture Human cells Leukemia Rotating wall vessel Stirred tank Transplant
This work was partially supported by CONACYT-Fondos Sectoriales Salud Grant 13993 and Grant I0010-2007-01, the sabbatical fellowship 79973 and PhD scholarship 103403.
- Bornstein R, Flores AI, Montalban MA, del Rey MJ, de la Serna J, Gilsanz F (2005) A modified cord blood collection method achieves sufficient levels for transplantation in most adult patients. Stem cells 23:324–334Google Scholar
- Introna M, Franceschetti M, Ciocca A, Borleri G, Conti E, Golay J, Rambaldi A (2006) Rapid and massive expansion of cord blood-derived cytokine-induced killer cells: an innovative proposal for the treatment of leukemia relapse after cord blood transplantation. Bone Marrow Transplant 38:621–627CrossRefGoogle Scholar
- Kasper C, Suck K, Anton F, Scheper T, Kall S, van Griensven M (2007) A newly developed rotating bed bioreactor for bone tissue engineering. In: Ashammakhi N, Reis R, Chiellini E (eds) Topics in tissue engineering. Oulu, Finland, pp 1–15Google Scholar
- Muller-Sieburg CE, Sieburg HB (2006) The GOD of hematopoietic stem cells, a clonal diversity model of the stem cell compartment. Cell Cycle 5:394–398Google Scholar
- Saulnier N, Di Campli C, Zocco MA, Di Gioacchino G, Novi M, Gasbarrini A (2005) From stem cell to solid organ. Bone marrow, peripheral blood or umbilical cord blood as favorable source? Eur Rev Med Pharmacol Sci 9:315–324Google Scholar
- Tong J, Gianni AM, Siena S, Srour EF, Bregni M, Hoffman R (1994) Primitive hematopoietic progenitor cells are present in peripheral blood autografts. Blood Cells 20:351–362, discussion 362–363Google Scholar
- Van Zant G, Rummel SA, Koller MR, Larson DB, Drubachevsky I, Palsson M, Emerson SG (1994) Expansion in bioreactors of human progenitor populations from cord blood and mobilized peripheral blood. Blood Cells 20:482–491Google Scholar
- Yamaguchi M, Hirayama F, Murahashi H, Azuma H, Sato N, Miyakazu H, Fukazawa K, Sawada K, Koike T, Kuwabara M, Ikeda H, Ikebuchi K (2002) Ex vivo expansion of UC blood primitive hematopoietic progenitors and transplantable stem cells using human primary BM stromal cells and human AB serum. Cytotherapy 4:109–118CrossRefGoogle Scholar
- Yoo ES, Lee KE, Seo JW, Yoo EH, Lee MA, Im SA, Mun YC, Lee SN, Huh JW, Kim MJ, Jo DY, Ahn JY, Lee SM, Chung WS, Kim JH, Seong CM (2003) Adherent cells generated during long-term culture of human cord blood CD34+ cells have characteristics of endothelial cells and beneficial effect on cord blood ex vivo expansion. Stem Cells 21:228–235CrossRefGoogle Scholar
- Zhai QL, Qiu LG, Li Q, Meng HX, Han JL, Herzig RH, Han ZC (2004) Short-term ex vivo expansion sustains the homing-related properties of umbilical cord blood hematopoietic stem and progenitor cells. Haematologica 89:265–272Google Scholar
- Zubler RH (2006) Ex vivo expansion of hematopoietic stem cells and gene therapy development. Swiss Med Wkly 136:795–799Google Scholar