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Enhancing effect of a protein from Lonomia obliqua hemolymph on recombinant protein production

  • SI:Heterologous Gene Expression
  • Published:
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Abstract

Gene expression in animal cells allows large scale production of proteins used for either structure and function studies or therapeutic purposes. Maximizing recombinant protein production is necessary to optimize cell growth and protein expression. Some studies have demonstrated the presence of pharmacologically active substances in insect hemolymph. In this work, we have identified and purified a protein from Lonomia obliqua hemolymph able to increase the production of the rabies virus glycoprotein, expressed in Drosophila melanogaster S2 cells, by about 59%.

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Abbreviations

FACS:

Fluorescence-assisted cell sorting

FBS:

Fetal bovine serum

rRVGP:

Recombinant rabies virus glycoprotein

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Acknowledgements

This work was supported by grants from FAPESP (02/09482-3 and 03/08728-1). We gratefully acknowledge the technical assistance provided by of Alexsander Seixas de Souza (Confocal) and the financial support of Fapesp: 02/09482-3; 03/08728-1

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Authors and Affiliations

  1. Laboratório de Imunologia Viral, Instituto Butantan, Av. Vital Brasil 1500, 05503-900, São Paulo, Brasil

    Ronaldo Z. Mendonça, Katia N. Greco, Alvaro P. B. Sousa, Renato M. Astray & Carlos A. Pereira

  2. Laboratório de Parasitologia, Instituto Butantan, São Paulo, Brasil

    Roberto H. P. Moraes

Authors
  1. Ronaldo Z. Mendonça
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  2. Katia N. Greco
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  3. Alvaro P. B. Sousa
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  4. Roberto H. P. Moraes
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  5. Renato M. Astray
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  6. Carlos A. Pereira
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Correspondence to Ronaldo Z. Mendonça.

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Mendonça, R.Z., Greco, K.N., Sousa, A.P.B. et al. Enhancing effect of a protein from Lonomia obliqua hemolymph on recombinant protein production. Cytotechnology 57, 83–91 (2008). https://doi.org/10.1007/s10616-008-9141-4

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  • DOI: https://doi.org/10.1007/s10616-008-9141-4

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