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Cephalic hedgehog expression is regulated directly by Sox17 in endoderm development of Xenopus laevis

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Abstract

In early development of animals, hedgehog (Hh) genes function as morphogen in the axis determination and the organ formation. In Xenopus, three hedgehog genes, sonic (shh), banded (bhh), and cephalic (chh), were identified and might organize various tissues and organs in embryogenesis. Here, we report the spatial and temporal regulation of Xchh which is expressed in endoderm cells differentiating to digestive organs. Xchh expression in endoderm was inhibited by ectopic expression of the dominant-negative activin receptor, tAR. Moreover, a maternally inherited transcription factor VegT and its downstream regulators activated Xchh expression. These indicates that Xchh is regulated by the factor involved in the cascade originated from VegT via activin/nodal signals. Using the Sox17α-VP16-GR construct, we showed that Xchh expression might be induced directly by transcription factor Sox17.

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Abbreviations

bFGF:

basic fibroblast growth factor

BMP:

bone morphogenic protein

TGFβ:

transforming growth factor β

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Acknowledgments

This research was supported by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan.

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Author notes

  1. Yuzuru Ito

    Present address: Center for Structuring Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo, 153-8902, Japan

Authors and Affiliations

  1. Graduate School of Systems Life Sciences, Kyushu University, Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Yumihiko Yagi, Satoru Kuhara & Kosuke Tashiro

  2. Graduate School of Genetic Resource Technology, Kyushu University, Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Yuzuru Ito, Satoru Kuhara & Kosuke Tashiro

Authors
  1. Yumihiko Yagi
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  2. Yuzuru Ito
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  4. Kosuke Tashiro
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Correspondence to Kosuke Tashiro.

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Yagi, Y., Ito, Y., Kuhara, S. et al. Cephalic hedgehog expression is regulated directly by Sox17 in endoderm development of Xenopus laevis . Cytotechnology 57, 151–159 (2008). https://doi.org/10.1007/s10616-008-9127-2

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  • DOI: https://doi.org/10.1007/s10616-008-9127-2

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