Abstract
In this study, we report on an in situ monitoring system of living cultured cells using infrared absorption spectroscopy in the geometry of multiple internal reflections (MIR-IRAS). In order to observe living cultured cells, the temperature in the sample chamber of a FT-IR spectrometer was maintained at 37 °C and a humidified gas mixture containing 5% CO2 was introduced into the sample chamber. Human breast cell line MCF-7 cultured on Si MIR prisms were placed in the sample chamber and infrared spectra of MCF-7 cells were collected for 5 h. It was found that the adhesion and metabolism of MCF-7 cells could be monitored by the absorption intensity of amide-II protein band (1,545 cm−1) and also by the absorption intensities of CH x bands (2,700–3,100 cm−1). These results suggest that our system is useful for a nondestructive and non-label monitoring of cell viability. Our method based on infrared absorption spectroscopy has a potential for bioscreening application.
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References
Emura K, Yamanaka S, Isoda H, Watanabe KN (2006) Estimation for different genotypes of plants based on DNA analysis using near-infrared (NIR) and fourier-transform infrared (FT-IR) spectroscopy. Breed sci 56:399–403
Gremlich H-U, Yan B (eds) (2001) Infrared and Raman Spectroscopy of biological materials. Marcel Dekker, Inc., New York
Han J, Isoda H, Maekawa T (2002) Analysis of the mechanism of the tight-junctional permeability increase by capsaicin treatment on the intestinal Caco-2 cells. Cytotechnology 40:93–98
Hutson TB, Mitchell ML, Keller JT, Long DJ, Chang MJW (1988) Mammalian cell growth studied in situ by fourier transform infrared spectroscopy. Anal Biochem 174:415–422
Isoda H, Talorete TPN, Kimura M, Maekawa T, Inamori Y, Nakajima N, Seki H (2002) Phytoestrogens genistein and daidzin enhance the acetylcholinesterase activity of the rat pheochromocytoma cell line PC12 by binding to the estrogen receptor. Cytotechnology 40:117–123
Mantsch HH, Chapman D (eds) (1996) Infrared spectroscopy of biomolecules. Eiley-Liss, Inc., New York
Miyamoto K, Ishibashi K, Hiroi K, Kimura Y, Ishii H, Niwano M (2005) Label-free detection and classification of DNA by surface vibration spectroscopy in conjugation with electrophoresis. Appl Phys Lett 86:053902
Miyamoto K, Ishibashi K, Yamaguchi R, Kimura Y, Ishii H, Niwano M (2006) In situ observation of DNA hybridization and denaturation by surface infrared spectroscopy. J Appl Phys 99:094702
Miyazawa T, Blout ER (1961) The infrared spectra of polypeptides in various conformations: amide I and II bands. J Am Chem Soc 83:712–719
Socrates G (2001) Infrared and Raman characteristic group frequencies. Wiley, pp 50–67 and pp 333–338
Talorete T, Isoda H, Maekawa T (2002) Agaricus blazei (Class Basidiomycotina) aqueous extract enhances the expression of c-jun protein in MCF-7 cells. J Agric Food Chem 50:5162–5166
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Part of this work was supported by Grants-in-Aid for Scientific Research (Grant No. 17206004) from the Japan Society for the Promotion of Science (JSPS).
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Miyamoto, Ki., Yamada, P., Yamaguchi, Rt. et al. In situ observation of a cell adhesion and metabolism using surface infrared spectroscopy. Cytotechnology 55, 143–149 (2007). https://doi.org/10.1007/s10616-007-9111-2
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DOI: https://doi.org/10.1007/s10616-007-9111-2