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Porcine platelet lysate as a supplement for animal cell culture

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Abstract

A novel supplementation of cell growth media based on a porcine platelet lysate was developed for culture of animal-derived cells. The platelet lysate was produced from porcine blood and contained lysate of platelets and plasma components. It showed satisfactory microbiological integrity and it carried only low amount of endotoxins (<10 EU/mL). The porcine platelet lysate supported well proliferation of Vero (African green monkey transformed kidney epithelial cells), Chinese hamster ovary (CHO) and hybridoma cells comparable to fetal bovine serum (FBS). Platelet lysate shows promise as a viable choice over FBS as it can be produced in large quantities, high lot-to-lot consistency and with an attractive price structure. Furthermore it is a strong alternative to FBS for ethical reasons. It is expected that it can be used as a general supplementation for most animal cells for research studies on the proliferation of cells and their expression of products.

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Abbreviations

FBS:

Fetal bovine serum

PDGF:

Platelet derived growth factor

TGF-β:

Transforming growth factor-β

BSE:

Bovine spongiform encephalopathy

DMEM:

Dulbecco’s Modified Eagle’s Medium

SEM:

Standard error of the mean

CHO:

Chinese hamster ovary

SFM:

Serum free media

EU:

Endotoxins units

PPL:

Porcine platelet lysate

References

  • Al-Rubeai M, Welzenbach K, Lloyd DR, Emery AN (1997) A rapid method for evaluation of cell number and viability by flow cytometry. Cytotechnology 24:161–168

    Article  Google Scholar 

  • Balk SD (1971) Calcium as a regulator of the proliferation of normal, but not transformed, chicken fibroblasts in a plasma-containing medium. Proc Natl Acad Sci USA 68(2):271–275

    Article  CAS  Google Scholar 

  • Butler M (2005) Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals. Appl Microbiol Biotechnol 68:283–291

    Article  CAS  Google Scholar 

  • Geerling G, Liu L, Harloff S, Wedel T, Hartwig D (2005) Platelet releasate: a potential treatment for ocular surface disease. The Ocular Surf 3:S64

    Google Scholar 

  • Jochems, CEA (1997) Use, trade and harvest of livestock sera. In Department of Laboratory Animal Sciences. Utrecht University, Utrecht, pp 1–64

  • Jochems CEA, van der Valk JBF, Stafleu FR, Baumans V (2002) The use of fetal bovine serum: ethical or scientific problem? ATLA- Alternatives to Laboratory Animals 30:219–227

    CAS  Google Scholar 

  • Johansson L, Klinth J, Holmqvist O, Ohlson S (2003) Platelet lysate: A Replacement for Fetal Bovine Serum in Animal Cell Culture. Cytotechnology 42:67–74

    Article  CAS  Google Scholar 

  • Lindeboom JAH, Mathura KR, Aartman IHA, Kroon FHM, Milstein DMJ, Ince C 2007. Influence of the application of platelet-enriched plasma in oral mucosal wound healing. Clin Oral Implant Res 18:133–139

    Google Scholar 

  • Merten O-W (2006) Introduction to animal cell culture technology–past, present and future. Cytotechnology 50:1–7

    Article  Google Scholar 

  • Persson A, Alfredsson N, Christensson K, Ohlson S, Holmqvist O (2006) Blood platelet lysate and method for producing the same. PCT application WO 2006/137778A1. Proliff AB

  • Ross R, Glomset J, Kariya B, Harker L (1974) A platelet-dependent serum factor that stimulates the proliferation of arterial smooth muscle cells in vitro. Proc Natl Acad Sci USA 71(4):1207–1210

    Article  CAS  Google Scholar 

  • van dert Valk J, Mellor D, Brands R, Fischer R, Gruber F, Gstraunthaler G, Hellebrekers L, Hyllner J, Jonker FH, Prieto P, Thalen M, Baumans V (2004) The Humane collection of fetal bovine serum and possibilities for serum-free cell and tissue culture. Toxicol In Vitro 18:1–12

    Article  Google Scholar 

  • Watatani S, Terashi H, Saigo K, Yokoyama M, Yamamoto M, Yokoo S, Komori T (2005) Autologous platelet-rich plasma (PRP) is more useful than fetal calf serum (FCS) in adipose-derived stem cells (ADSCS) culture for bone regeneration. Int J Oral Maxillofac Surg 34:123

    Article  Google Scholar 

  • Whitford WG (2005) Supplementation of animal cell culture media. BioProcess Int 3:28–36

    Google Scholar 

Download references

Acknowledgements

Financial support from the University of Kalmar, Ellco Food AB, ProLiff AB and the Knowledge Foundation is gratefully acknowledged.

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Authors and Affiliations

  1. School of Pure and Applied Natural Sciences, University of Kalmar, SE-391 82, Kalmar, Sweden

    Anna Aldén, Lorena Gonzalez & Sten Ohlson

  2. Ellco Food, P. O. Box 100, SE-244 22, Kävlinge, Sweden

    Anna Persson & Kerstin Christensson

  3. ProLiff AB, SE-392 35, Kalmar, Sweden

    Olov Holmqvist

Authors
  1. Anna Aldén
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  2. Lorena Gonzalez
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  3. Anna Persson
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  4. Kerstin Christensson
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  5. Olov Holmqvist
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  6. Sten Ohlson
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Corresponding author

Correspondence to Sten Ohlson.

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Aldén, A., Gonzalez, L., Persson, A. et al. Porcine platelet lysate as a supplement for animal cell culture. Cytotechnology 55, 3–8 (2007). https://doi.org/10.1007/s10616-007-9097-9

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  • DOI: https://doi.org/10.1007/s10616-007-9097-9

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