, Volume 54, Issue 2, pp 115–120 | Cite as

Treatment of long tubular bone defect of rabbit using autologous cultured osteoblasts mixed with fibrin

  • Seok-Jung Kim
  • Jae-Deog Jang
  • Seung-Koo Lee
Original Research


The osteogenic potential of autologous cultured osteoblasts mixed with fibrin when transplanted to bone defects was evaluated. Radial shaft defects over 15 mm were made in 30 New Zealand white rabbits. A total of 15 rabbits in the control group underwent an iliac bone graft and 15 rabbits in the experimental group underwent an autologous cultured osteoblast injection mixed with fibrin. Both groups were compared radiologically and 5 rabbits in each group were sacrificed for histological evaluation using H-E and Masson’s trichrome stain at 3, 6, and 9 weeks. Osteogenesis in the control group progressed more rapidly than in the experimental group. However, at 9 weeks, bone formation in both groups were similar and showed no significant difference in terms of the amount of bone formation and the quality of bone union. Autologous cultured osteoblast transplantation mixed with fibrin in bone defects was found to produce bone efficiently.


Osteoblast Fibrin Osteogenesis Autologous transplantation 


  1. Ahlmann E, Patzakis M, Roidis N (2002) Comparison of anterior and posterior iliac crest bone grafts in terms of harvest-site morbidity and functional outcomes. J Bone Joint Surg 84-A:716–720Google Scholar
  2. Ashton BA, Allen TD, Howlett CR, Eaglesom CC, Hattori A, Owen M (1980) Formation of bone and cartilage by marrow stromal cells in diffusion chambers in vivo. Clin Orthop 151:294–307Google Scholar
  3. Bos GD, Goldberg VM, Powell AE, Heiple KG, Zika JM (1983) The effect of histocompatibility matching on canine frozen bone allografts. J Bone Joint Surg 65-A:89–96 Google Scholar
  4. Bruder SP, Fink DJ, Caplan AI (1994) Mesenchymal stem cells in bone development, bone repair, and skeletal regeneration therapy. J Cell Biochem 56:283–294CrossRefGoogle Scholar
  5. Bruder SP, Jaiswal N, Haynesworth SE (1997) Growth kinetics, self-renewal and the osteogenic potentials of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation. J Cell Bioch 64:278–294CrossRefGoogle Scholar
  6. Cheng SL, Yang JW, Rifas L, Zhang SF, Avioli LV (1994) Differentiation of human bone marrow osteogenic stromal cells in vitro: induction of the osteoblast phenotype by dexamethasone. Endocrinology 134:277–286CrossRefGoogle Scholar
  7. Herold HZ, Hurvitz A, Tadmor A (1971) The effect of growth hormone on the healing of experimental bone defects. Acta Orthop Scand 42:377–384CrossRefGoogle Scholar
  8. Huang Q, Goh JC, Hutmacher DW, Lee EH (2002) In vivo mesenchymal cell recruitment by a scaffold loaded with transforming growth factor beta1 and the potential for in situ chondrogenesis. Tissue Eng 8:469–482CrossRefGoogle Scholar
  9. Liu J, Wang Z, Hu Y (2000) Complications of massive allografts after segmental resection of malignant bone tumors. Zhongh ua Wai Ke Za Zhi 38:332–335Google Scholar
  10. Maniatopoulos C, Sodek J, Melcher AH (1988) Bone formation in vitro by stromal cells obtained from bone marrow of young adults rat. Cell Tissue Res 254:317–330CrossRefGoogle Scholar
  11. Muschler GF, Boehm C, Easley K (1997) Aspiration to obtain osteoblast progenitor cells from human bone marrow: the influence of aspiration volume. J Bone Joint Surg 79-A:1699–1709Google Scholar
  12. Pittenger MF, Mackay AM, Beck SC (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284:143–147CrossRefGoogle Scholar
  13. Schneider U (1998) Autogenous bone cell transplantation. Orthopade 27:143–146Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Department of Orthopedic SurgeryThe Catholic University of Korea, College of MedicineSeoulKorea
  2. 2.Department of Orthopedic SurgeryUijeongbu St. Mary’s HospitalUijeongbu CityKorea
  3. 3.Central Research InstituteSewonCellontechSeongsu-dongKorea

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