, Volume 53, Issue 1–3, pp 3–22 | Cite as

Using cell engineering and omic tools for the improvement of cell culture processes

  • Darrin Kuystermans
  • Britta Krampe
  • Halina Swiderek
  • Mohamed Al-Rubeai
NICB special issue


Significant strides have been made in mammalian cell based biopharmaceutical process and cell line development over the past years. With several established mammalian host cell lines and expression systems, optimization of selection systems to reduce development times and improvement of glycosylation patterns are only some of the advances being made to improve cell culture processes. In this article, the advances pertaining to cell line development and cell engineering strategies are discussed. An overview of the cell engineering strategies to enhance cellular characteristics by genetic manipulation are illustrated, focusing on the use of genomics and proteomics tools and their application in such endeavors. Included in this review are some of the early studies using the ‘omic’ technique to understand cellular mechanisms of product synthesis and secretion, apoptosis, cell proliferation and the influence of the physicochemical environment. The article highlights the significance of integrating genomics and proteomics data with the vast amounts of bioprocess data for improved analysis of the biological pathways involved. Further improvements of the techniques and methodologies used are needed but ultimately, the new cell engineering strategies should provide great insight into the regulatory networks within the cell in a bioprocess environment and how to manipulate them to increase overall productivity.


Cell engineering Proteomics Genomics Bioinformatics Biopharmaceuticals 



This work is funded by Science Foundation Ireland (SFI).


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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • Darrin Kuystermans
    • 1
  • Britta Krampe
    • 1
  • Halina Swiderek
    • 1
  • Mohamed Al-Rubeai
    • 1
  1. 1.School of Chemical and Bioprocess Engineering and Centre for Synthesis and Chemical BiologyUniversity College DublinDublin 4Ireland

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