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Cytotechnology

, Volume 52, Issue 2, pp 113–124 | Cite as

Growth, metabolism and baculovirus production in suspension cultures of an Anticarsia gemmatalis cell line

  • Verónica Viviana Gioria
  • Volker Jäger
  • Juan Daniel ClausEmail author
Original Research

Abstract

The UFL-AG-286 cell line, established from embryonic tissue of the lepidopteran insect Anticarsia gemmatalis, has been identified as a good candidate to be used as a cellular substrate in the development of a process for in vitro production of the Anticarsia gemmatalis multicapsid nucleopolyhedrovirus, a baculovirus widely used as bioinsecticide. In order to characterize the technological properties of this cell line and evaluate its feasibility to use it for the large-scale production of Anticarsia gemmatalis multicapsid nucleopolyhedrovirus, UFL-AG-286 cells were adapted to grow as agitated suspension cultures in spinner-flasks. Batch suspension cultures of adapted cells in serum-supplemented TC-100 medium grew with a doubling time of about 29 h and reached a maximum cell density higher than 3.5 × 106 viable cells ml−1. At the end of the growth period glucose was completely depleted from the culture medium, but l-lactate was not produced. Amino acids, with the exception of glutamine, were only negligibly consumed or produced. In contrast to other insect cell lines, UFL-AG-286 cells appeared to be unable to synthesize alanine as a metabolic way to dispose the by-product ammonia. The synchronous infection of suspension cultures with Anticarsia gemmatalis multicapsid nucleopolyhedrovirus in the early to medium exponential growth phase yielded high amounts of both viral progenies per cell and reduced the specific demands of UFL-AG-286 cells for the main nutrients.

Keywords

Anticarsia gemmatalis Baculovirus Cell suspension culture Lepidopteran insect cell line UFL-AG-286 AgMNPV 

Abbreviations

AgMNPV

Anticarsia gemmatalis multicapsid nucleopolyhedrovirus

NOV

non-occluded virus

PIB

polyhedral inclusion body

TCID50%

tissue culture infectious dose 50%

Notes

Acknowledgements

The authors gratefully acknowledge support from ANPCyT (PICT 08-04411 BID 802 OC/AR) and Universidad Nacional del Litoral (CAI + D 2002).

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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • Verónica Viviana Gioria
    • 1
  • Volker Jäger
    • 2
  • Juan Daniel Claus
    • 1
    Email author
  1. 1.Laboratorio de Virología, Facultad de Bioquímica y Ciencias BiológicasUniversidad Nacional del Litoral, CC 242Santa FeRepública Argentina
  2. 2.Helmholtz Zentrum für Infektionsforschung GmbHBraunschweigGermany

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