, Volume 52, Issue 2, pp 107–112 | Cite as

A simple and fast method for cell recovery and DNA content analysis from various mouse tissues by flow cytometry

  • Laurent Foiry
  • Jérôme Mégret
  • Claudine Junien
  • Geneviève GourdonEmail author
Original Paper


Cell division in tissues can be investigated in various ways. We present here a method for improving cell recovery and cell cycle analysis for a wide range of mouse tissues. This strategy combines a cell isolation procedure for various mouse tissues based on intracardiac perfusion and subsequent treatment followed by flow cytometry. This easy and reproducible method allows a rapid analysis of nuclear DNA content, providing an estimate of the cell number at different phases of the cell cycle. This combined procedure could also be used for the isolation of specific cell subpopulations from different mouse tissues by fluorescence activated cell sorting.


Cell cycle Cell isolation Flow Cytometry Mouse Tissue dissociation 


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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Laurent Foiry
    • 1
  • Jérôme Mégret
    • 2
  • Claudine Junien
    • 1
  • Geneviève Gourdon
    • 1
    Email author
  1. 1.Inserm U781Clinique M. Lamy, Hôpital Necker Enfants MaladesParisFrance
  2. 2.Service de Tri CellulaireInstitut de Recherche Necker Enfants Malades, Faculté de Médecine NeckerParisFrance

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