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Cytotechnology

, Volume 52, Issue 2, pp 107–112 | Cite as

A simple and fast method for cell recovery and DNA content analysis from various mouse tissues by flow cytometry

  • Laurent Foiry
  • Jérôme Mégret
  • Claudine Junien
  • Geneviève GourdonEmail author
Original Paper

Abstract

Cell division in tissues can be investigated in various ways. We present here a method for improving cell recovery and cell cycle analysis for a wide range of mouse tissues. This strategy combines a cell isolation procedure for various mouse tissues based on intracardiac perfusion and subsequent treatment followed by flow cytometry. This easy and reproducible method allows a rapid analysis of nuclear DNA content, providing an estimate of the cell number at different phases of the cell cycle. This combined procedure could also be used for the isolation of specific cell subpopulations from different mouse tissues by fluorescence activated cell sorting.

Keywords

Cell cycle Cell isolation Flow Cytometry Mouse Tissue dissociation 

References

  1. Burns ER, Bagwell CB, Hinson WG, Pipkin JL Jr, Hudson JL (1983) Preparation and stability of sixteen murine tissues and organs for flow cytometric cell cycle analysis. Cytometry 4(2):150–160CrossRefGoogle Scholar
  2. Cram LS (2002) Flow cytometry, an overview. Methods Cell Sci 24(1–3):1–9CrossRefGoogle Scholar
  3. deFazio A, Leary JA, Hedley DW, Tattersall MH (1987) Immunohistochemical detection of proliferating cells in vivo. J Histochem Cytochem 35(5):571–577Google Scholar
  4. Edstrom S, Ekman L, Ternell M, Lundholm K (1983) Isolation of mouse liver cells: perfusion technique and metabolic evaluation. Eur Surg Res 15(2):97–102Google Scholar
  5. Krishan A (1975) Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining. J Cell Biol 66(1):188–193CrossRefGoogle Scholar
  6. Messier B, Leblond CP (1960) Cell proliferation and migration as revealed by radioautography after injection of thymidine-H3 into male rats and mice. Am J Anat 106:247–285Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Laurent Foiry
    • 1
  • Jérôme Mégret
    • 2
  • Claudine Junien
    • 1
  • Geneviève Gourdon
    • 1
    Email author
  1. 1.Inserm U781Clinique M. Lamy, Hôpital Necker Enfants MaladesParisFrance
  2. 2.Service de Tri CellulaireInstitut de Recherche Necker Enfants Malades, Faculté de Médecine NeckerParisFrance

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