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Cytotechnology

, Volume 52, Issue 2, pp 99–106 | Cite as

Development of porous collagen beads for chondrocyte culture

  • Tracy A. Tebb
  • Shiao-Wen Tsai
  • Veronica Glattauer
  • Jacinta F. White
  • John A. M. Ramshaw
  • Jerome A. WerkmeisterEmail author
Original Paper

Abstract

A method for the preparation of bioresorbable collagen beads with an open porous structure is presented. These beads were prepared from collagen-alginate composite beads by removal of the alginate component. These collagen beads were suitable for rapid proliferation of chondrocytes in a dynamic, spinner culture system. Histology and immuno-histology showed that biochemical markers of chondrocytes are present during this cell proliferation, indicating that there was control of phenotype and that cell de-differentiation had not occurred. Unlike other 3-D scaffolds that have been used, the cells were amplified from very low cell densities and were able to proliferate freely without loss of phenotype.

Keywords

Collagen beads Alginate Cell culture Chondrocytes Tissue engineering 

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Tracy A. Tebb
    • 1
  • Shiao-Wen Tsai
    • 2
    • 3
  • Veronica Glattauer
    • 1
  • Jacinta F. White
    • 1
  • John A. M. Ramshaw
    • 1
  • Jerome A. Werkmeister
    • 1
    Email author
  1. 1.CSIRO, Molecular and Health TechnologiesClayton SouthAustralia
  2. 2.ITRI, Biomedical Engineering CenterHsinchuTaiwan, R.O.C.
  3. 3.Institute of Biochemical and Biomedical EngineeringChang-Gung UniversityTaoyuanTaiwan, R.O.C.

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