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Development of porous collagen beads for chondrocyte culture

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Abstract

A method for the preparation of bioresorbable collagen beads with an open porous structure is presented. These beads were prepared from collagen-alginate composite beads by removal of the alginate component. These collagen beads were suitable for rapid proliferation of chondrocytes in a dynamic, spinner culture system. Histology and immuno-histology showed that biochemical markers of chondrocytes are present during this cell proliferation, indicating that there was control of phenotype and that cell de-differentiation had not occurred. Unlike other 3-D scaffolds that have been used, the cells were amplified from very low cell densities and were able to proliferate freely without loss of phenotype.

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Author notes

  1. Shiao-Wen Tsai

    Present address: Institute of Biochemical and Biomedical Engineering, Chang-Gung University, Taoyuan, Taiwan, R.O.C.

Authors and Affiliations

  1. CSIRO, Molecular and Health Technologies, Bayview Avenue, Clayton South, VIC, 3169, Australia

    Tracy A. Tebb, Veronica Glattauer, Jacinta F. White, John A. M. Ramshaw & Jerome A. Werkmeister

  2. ITRI, Biomedical Engineering Center, 195-85 Chung Hsing Road, Hsinchu, 310, Taiwan, R.O.C.

    Shiao-Wen Tsai

Authors
  1. Tracy A. Tebb
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  2. Shiao-Wen Tsai
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  3. Veronica Glattauer
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  4. Jacinta F. White
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  5. John A. M. Ramshaw
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  6. Jerome A. Werkmeister
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Corresponding author

Correspondence to Jerome A. Werkmeister.

Additional information

Tracy A. Tebb, Shiao-Wen Tsai, Veronica Glattauer and Jacinta F. White have made equal contributions to this study.

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Tebb, T.A., Tsai, SW., Glattauer, V. et al. Development of porous collagen beads for chondrocyte culture. Cytotechnology 52, 99–106 (2006). https://doi.org/10.1007/s10616-006-9034-3

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  • DOI: https://doi.org/10.1007/s10616-006-9034-3

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