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Insulin secretion dynamics of free and alginate-encapsulated insulinoma cells

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Abstract

This study investigates the effect of alginate/poly-l-lysine/alginate (APA) encapsulation on the insulin secretion dynamics exhibited by an encapsulated cell system. Experiments were performed with the aid of a home-built perfusion apparatus providing a 1 min temporal resolution. Insulin profiles were measured from: (i) murine insulinoma βTC3 cells encapsulated in calcium alginate/poly-l-lysine/alginate (APA) beads generated with high guluronic (G) or high mannuoric (M) content alginate, and (ii) murine insulinoma βTC-tet cells encapsulated in high M APA beads and propagated in the presence and absence of tetracycline. Results show that encapsulation in APA beads did not affect the insulin secretion profile shortly post-encapsulation. However, remodeling of the beads due to cell proliferation affected the insulin secretion profiles; and inhibiting remodeling by suppressing cell growth preserved the secretion profile. The implications of these findings regarding the in vivo function of encapsulated insulin secreting cells are discussed.

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Acknowledgements

This work was supported by funding from NIH (DK 56980) through a subcontract to Georgia Tech, as well as from the Georgia Tech/Emory Center (GTEC) for the Engineering of Living Tissues, an ERC Program of the National Science Foundation under Award Number EEC-9731643. This financial support is greatly appreciated. Additionally, the authors thank Dr. S. Efrat for providing the βTC3 and βTC-tet cells and Tracey Couse for help with histology.

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  1. Shing-Yi Cheng

    Present address: Novocell Inc., 31 Technology Drive, Irvine, CA, 92618, USA

Authors and Affiliations

  1. Chemical & Biomolecular Engineering, Georgia Institute of Technology, 315 Ferst Drive, IBB Building, Room 1306, Atlanta, GA, 30332, USA

    Shing-Yi Cheng, Ioannis Constantinidis & Athanassios Sambanis

  2. Division of Endocrinology, Department of Medicine, University of Florida, Gainesville, FL, 32610, USA

    Ioannis Constantinidis

Authors
  1. Shing-Yi Cheng
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  2. Ioannis Constantinidis
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  3. Athanassios Sambanis
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Correspondence to Athanassios Sambanis.

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Cheng, SY., Constantinidis, I. & Sambanis, A. Insulin secretion dynamics of free and alginate-encapsulated insulinoma cells. Cytotechnology 51, 159–170 (2006). https://doi.org/10.1007/s10616-006-9025-4

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  • DOI: https://doi.org/10.1007/s10616-006-9025-4

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