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YY1 binds to regulatory element of chicken lysozyme and ovalbumin promoters

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Abstract

Chicken lysozyme is highly expressed in the oviduct. The 5′ regulatory region of this gene contains a negative element that represses transcription. To assess the molecular basis underlying the regulation of lysozyme gene expression, we investigated the binding protein to this region. Sequence motif analysis suggested the existence of putative YY1 binding sites in this regulatory region. Electrophoretic mobility shift assay showed the specific binding of YY1 to the negative element. In addition, chromatin immunoprecipitation assay indicated that YY1 specifically bound to the negative element in oviduct cells but not in erythrocytes. It was suggested by electrophoretic mobility shift assay and chromatin immunoprecipitation assay that YY1 also bound to the negative regulatory region in the promoter of the ovalbumin gene which also shows oviduct-specific expression. Western blot analysis showed that YY1 was expressed in relatively high levels in the oviduct and nucleus fractionation experiments showed that YY1 was localized both in chromosome and nuclear matrix fractions. These results suggest that there are some specific roles in the negative regulatory regions of these genes in relation to the multifunctional transcription factor YY1.

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Abbreviations

ChIP:

chromatin immunoprecipitation

DHS:

DNase I-hypersensitive site

EMSA:

electrophoretic mobility shift assay

NE:

negative element

NRE:

negative regulatory element

SDRE:

steroid dependent regulatory element

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Acknowledgements

This work was supported in part by a Grant-in-Aid for Young Scientists (B) (16760634) from the Ministry of Education, Science, Sports and Culture of Japan and a Grant-in-Aid for Scientific Research (17360396) from Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Department of Biotechnology, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan

    Mahboob Morshed, Munetoshi Ando, Junko Yamamoto, Akitsu Hotta, Hidenori Kaneoka, Jun Kojima, Ken-ichi Nishijima & Shinji Iijima

  2. Department of Chemical Engineering, Faculty of Engineering, Kyushu University, Motooka, Nishi-ku, Fukuoka, 819-0395, Japan

    Masamichi Kamihira

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  1. Mahboob Morshed
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  2. Munetoshi Ando
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  3. Junko Yamamoto
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  4. Akitsu Hotta
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  5. Hidenori Kaneoka
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  6. Jun Kojima
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  7. Ken-ichi Nishijima
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  8. Masamichi Kamihira
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  9. Shinji Iijima
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Correspondence to Ken-ichi Nishijima.

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Morshed, M., Ando, M., Yamamoto, J. et al. YY1 binds to regulatory element of chicken lysozyme and ovalbumin promoters. Cytotechnology 52, 159–170 (2006). https://doi.org/10.1007/s10616-006-9017-4

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  • DOI: https://doi.org/10.1007/s10616-006-9017-4

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