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Cytotechnology

, Volume 49, Issue 1, pp 71–75 | Cite as

An Inexpensive Method for Applying Nitrogen Evaporation to Hexane-containing 24- or 96-well Plates

  • Carrie P. Nevins
  • Janet L. Vierck
  • Lindsey D. Bogachus
  • Nicole S. Velotta
  • Federico Castro-Munozledo
  • Michael V. Dodson
Article

Abstract

A method is described for assembling an evaporation manifold from a cell culture flask, which allows for efficient nitrogen evaporation of hexane from 24- and 96-well plates. The precursor parts are readily available in most research laboratories. The nitrogen evaporation manifold is inexpensive, could possibly be used with other organic solvents, and appears ideal for a small number of samples in a multi-well format.

Keywords

Nitrogen drying Solvent evaporation 

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References

  1. Komarek, R.J. 1967Device for evaporation of solvent from small samplesJ. Lipid Res.8287288PubMedGoogle Scholar
  2. Ramirez-Zacarias, J.L., Castro-Munozledo, F., Kuri-Harcuch, W. 1992Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red OHistochemistry97493497PubMedGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Carrie P. Nevins
    • 1
  • Janet L. Vierck
    • 1
  • Lindsey D. Bogachus
    • 1
  • Nicole S. Velotta
    • 1
  • Federico Castro-Munozledo
    • 2
  • Michael V. Dodson
    • 1
  1. 1.Muscle Biology Laboratory, Department of Animal SciencesWashington State UniversityPullmanUSA
  2. 2.Department of Cell BiologyCINVESTAVMexico CityMexico

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