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A Simple and Quick Method to Isolate Nuclear Extracts from Pupae of Drosophila melanogaster.

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Abstract

Preparation of nuclear extracts is a critical step for biochemical identification of factors which function in the nuclei such as transcription factors. We have established a new method to prepare nuclear extracts from prepupae or pupae of Drosophila melanogaster. The method is simple and particularly useful for small-scale isolation from materials that are hard to get in large amounts such as specific tissues or animal bodies covered by pupal cases.

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Authors and Affiliations

  1. Advanced Research Institute for Science and Engineering, Waseda University, Higashi-Fushimi, Nishitokyo, 202-0021, Japan

    Haruhisa Kawasaki

  2. Department of Developmental Genetics, National Institute of Genetics, Mishima, 411-8540, Japan

    Susumu Hirose

  3. The Faculty of Science & The Graduate School of Science, Okayama University, Okayama, 700-8530, Japan

    Hitoshi Ueda

  4. The Graduate School of Natural Science and Technology, and Department of Biology, Faculty of Science, Okayama University, 3-1-1 Tsushima-naka, Okayama, 700-8530, Japan

    Hitoshi Ueda

Authors
  1. Haruhisa Kawasaki
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  2. Susumu Hirose
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  3. Hitoshi Ueda
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Correspondence to Hitoshi Ueda.

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Kawasaki, H., Hirose, S. & Ueda, H. A Simple and Quick Method to Isolate Nuclear Extracts from Pupae of Drosophila melanogaster.. Cytotechnology 49, 67–70 (2005). https://doi.org/10.1007/s10616-005-5414-3

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  • DOI: https://doi.org/10.1007/s10616-005-5414-3

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