Abstract
Selection of appropriate cultures having an osteogenic potential is a necessity if cell/biomaterial interactions are studied in long-term cultures. Osteoblastic cells derived from rat long bones or calvaria have the disadvantage of being in an advanced differentiation stage which results in terminal differentiation within 21 days. In this regard, less differentiated periosteum-derived osteoprogenitors could be more suitable.
Periosteum-derived cells were isolated from the tibiae of adult Wistar rats (n = 12). The osteogenic potential with regard to alkaline phosphatase activity, morphology, nodule formation and mineralization was studied by culturing them in an osteogenic medium for up to 4 months.
Seventy-five percent of the cultures (n = 9) did not show any increase in alkaline phosphatase activity nor nodule formation during long-term culture for up to 4 months. Nevertheless, in 25% of the cultures, alkaline phosphatase activity started from negligible (<5 mM pNP/mg protein) and increased towards approximately 50 mM pNP/mg protein. Three-dimensional nodule formation was observed at passages 3–5. In further passages (P5–P7), nodule formation capacity decreased and a diffuse mineralization pattern was observed.
Suitable cultures with osteogenic capacity, can be selected at early passages based on the presence of cuboidal cells. These cells have the advantage of retaining their osteogenic potential even after prolonged cultivation (6–7 passages) before final differentiation occurs. Although periosteal cells are suitable for long term in vitro evaluation of biomaterials, the isolation and selection is time consuming. Hence, a more appropriate source to study cell/biomaterial interactions should be more convenient.
Similar content being viewed by others
References
U. Arnold K. Lindenhayn C. Perka (2002) ArticleTitleIn vitro-cultivation of human periosteum derived cells in bioresorbable polymer-TCP-composites Biomaterials 23 2303–2310 Occurrence Handle10.1016/S0142-9612(01)00364-7 Occurrence Handle1:CAS:528:DC%2BD38Xitlaktb4%3D Occurrence Handle12013177
M.A. Attawia J.E. Devin C.T. Laurencin (1995) ArticleTitleImmunofluorescence and confocal laser scanning microscopy studies of osteoblast growth and phenotypic expression in three-dimensional degradable matrices J. Biomed. Mater. Res. 29 843–848 Occurrence Handle1:CAS:528:DyaK2MXms1WlsL0%3D Occurrence Handle7593023
S. Bahrami U. Stratmann H.P. Wiesmann K. Mokrys P. Bruckner T. Szuwart (2000) ArticleTitlePeriosteally derived osteoblast-like cells differentiate into chondrocytes in suspension culture in agarose Anat. Rec. 259 IssueID2 124–130 Occurrence Handle10.1002/(SICI)1097-0185(20000601)259:2<124::AID-AR2>3.0.CO;2-O Occurrence Handle1:STN:280:DC%2BD3c3otFamtA%3D%3D Occurrence Handle10820314
P. Böck (1984) Der Semidünnschnitt J.F. Bergmann Verlag München 98–99
A.S. Breitbart D.A. Grande R. Kessler J.T. Ryaby R.J. Fitzsimmons R.T. Grant (1998) ArticleTitleTissue engineered bone repair of calvarial defects using cultured periosteal cells Plast. Reconstr. Surg. 101 567–576 Occurrence Handle1:STN:280:DyaK1c7mtVSjuw%3D%3D Occurrence Handle9500373
J.A. Burdick R.F. Padera J.V. Huang K.S. Anseth (2002) ArticleTitleAn investigation of the cytotoxicity and histocompatibility of in situ forming lactic acid based orthopedic biomaterials J. Biomed. Mater. Res. (Appl. Biomater.) 63 484–491 Occurrence Handle1:CAS:528:DC%2BD38XnsFKmtrY%3D
L. Calandrelli B. Immirzi M. Malinconico G. Orsello M.G. Volpe F. Della Ragione V. Zappia (2002) ArticleTitleBiocompatibility studies on biodegradable polyester-based composites of human osteoblasts: a preliminary screening J. Biomed. Mater. Res. 59 611–617 Occurrence Handle10.1002/jbm.10014 Occurrence Handle1:CAS:528:DC%2BD38XksFCjtw%3D%3D Occurrence Handle11774322
M.J. Dalby L. Silvio ParticleDi E.J. Harper W. Bonfield (2002) ArticleTitleIncreasing hydroxyapatite incorporation into poly(methylmethacrylate) cement increases osteoblast adhesion and response Biomaterials 23 569–576 Occurrence Handle1:CAS:528:DC%2BD3MXosFKnu7g%3D Occurrence Handle11761177
C. Bari ParticleDe F. Dell’Accio F.P. Luyten (2001) ArticleTitleHuman periosteum-derived cells maintain phenotypic stability and chondrogenic potential throughout expansion regardless of donor age Arthritis Rheum. 44 IssueID1 85–95 Occurrence Handle11212180
H. Declercq N. Vreken ParticleVan den E. Maeyer ParticleDe R. Verbeeck E. Schacht L. Ridder ParticleDe R. Cornelissen (2004) ArticleTitleIsolation, proliferation and differentiation of osteoblastic cells to study cell/biomaterial interactions: comparison of different isolation techniques and source Biomaterials 25 757–768 Occurrence Handle10.1016/S0142-9612(03)00580-5 Occurrence Handle1:CAS:528:DC%2BD3sXoslOntLc%3D Occurrence Handle14609664
H.A. Declercq R.M.H. Verbeeck L.I.F.J.M. Ridder ParticleDe E.H. Schacht M.J. Cornelissen (2005) ArticleTitleCalcification as an indicator of osteoinductive capacity of biomaterials in osteoblastic cell cultures Biomaterials 26 4964–4974 Occurrence Handle10.1016/j.biomaterials.2005.01.025 Occurrence Handle1:CAS:528:DC%2BD2MXitlSns70%3D Occurrence Handle15769532
F.C.M. Driessens R.M.H. Verbeeck (1990) Biominerals CRC Press Boca Raton
K. Furuya A. Nifuji V. Rosen M. Noda (1999) ArticleTitleEffects of GDF7/BMP12 on proliferation and alkaline phosphatase expression in rat osteoblastic osteosarcoma ROS 17/2.8 cells J. Cell Biochem. 72 177–180 Occurrence Handle10.1002/(SICI)1097-4644(19990201)72:2<177::AID-JCB2>3.0.CO;2-# Occurrence Handle1:CAS:528:DyaK1MXjt1Oqsw%3D%3D Occurrence Handle10022500
R. Gruber C. Mayer K. Bobacz M. Krauth W. Graninger F.P. Luyten L. Erlacher (2001) ArticleTitleEffects of cartilage-derived morphogenetic proteins and osteogenic protein-1 on osteochondrogenic differentiation of periosteum-derived cells Endocrinology 142 2087–2094 Occurrence Handle10.1210/en.142.5.2087 Occurrence Handle1:CAS:528:DC%2BD3MXjt1aku78%3D Occurrence Handle11316776
K. Hanada L.A. Solchaga A.I. Caplan T.M. Hering V.M. Goldberg J.U. Yoo B. Johnstone (2001) ArticleTitleBMP-2 induction and TGF-beta1 modulation of rat periosteal cell chondrogenesis J. Cell Biochem. 81 284–294 Occurrence Handle10.1002/1097-4644(20010501)81:2<284::AID-JCB1043>3.0.CO;2-D Occurrence Handle1:CAS:528:DC%2BD3MXivVKgsbw%3D Occurrence Handle11241668
S.L. Ishaug G.M. Crane M.J. Miller A.W. Yasko M.J. Yasemski A.G. Mikos (1997) ArticleTitleBone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffolds J. Biomed. Mater. Res. 37 17–28
M. Iwasaki H. Nakahara T. Nakase T. Kimura K. Takaoka A.I. Caplan K. Ono (1994) ArticleTitleBone morphogenetic protein 2 stimulates osteogenesis but does not affect chondrogenesis in osteochondrogenic differentiation of periosteum-derived cells J. Bone Miner. Res. 9 IssueID8 1195–1204 Occurrence Handle1:CAS:528:DyaK2cXmvFemtL8%3D Occurrence Handle7976502
M. Iwasaki H. Nakahara K. Nakata T. Nakase T. Kimura K. Ono (1995) ArticleTitleRegulation of proliferation and osteochondrogenic differentiation of periosteum-derived cells by transforming growth factor-β and basic fibroblast growth factor J. Bone Joint Surg. 77-A IssueID4 543–554
T. Izumi S.P. Scully A. Heydemann M.E. Bolander (1992) ArticleTitleTransforming growth factor β1 stimulates type II collagen expression in cultured periosteum-derived cells J. Bone Miner. Res. 7 IssueID1 115–121 Occurrence Handle1:CAS:528:DyaK38XhsVCkt70%3D Occurrence Handle1549955
Y. Koshihara M. Kawamura S. Endo C. Tsutsumi H. Kodama H. Oda S. Higaki (1989) ArticleTitleEstablishment of human osteoblastic cells derived from periosteum in culture In Vitro Cell Dev. Biol. 25 IssueID1 37–43 Occurrence Handle1:STN:280:BiaC383psVA%3D Occurrence Handle2783688
Y. Koshihara M. Kawamura H. Oda S. Higaki (1987) ArticleTitleIn vitro calcification in human osteoblastic cell line derived from periosteum Biochem. Biophys. Res. Commun. 145 IssueID2 651–657 Occurrence Handle10.1016/0006-291X(87)91014-X Occurrence Handle1:CAS:528:DyaL2sXksFKgtLY%3D Occurrence Handle3593362
G. Lisignoli N. Zino G. Remiddi A. Piacentini A. Puggioli C. Trimarchi M. Fini N.M. Maraldi A. Facchini (2001) ArticleTitleBasic fibroblast growth factor enhances in vitro mineralization of rat bone marrow stromal cells grown on non-woven hyaluronic acid based polymer scaffold Biomaterials 22 2095–2105 Occurrence Handle10.1016/S0142-9612(00)00398-7 Occurrence Handle1:CAS:528:DC%2BD3MXlt1Kmt7Y%3D Occurrence Handle11432589
A.M. Moursi A.V. Winnard J.J. Lannutti R. Seghi (2002) ArticleTitleEnhanced osteoblast response to a polymethylmethacrylate–hydroxyapatite composite Biomaterials 23 133–144 Occurrence Handle10.1016/S0142-9612(01)00088-6 Occurrence Handle1:CAS:528:DC%2BD3MXosFKntrY%3D Occurrence Handle11762831
C. Perka O. Schultz R. Spitzer K. Lindenhayn G. Burmester M. Sittinger (2000) ArticleTitleSegmental bone repair by tissue- engineered periosteal cell transplants with bioresorbable fleece and fibrin scaffolds in rabbits Biomaterials 21 1145–1153 Occurrence Handle10.1016/S0142-9612(99)00280-X Occurrence Handle1:CAS:528:DC%2BD3cXjtFWls74%3D Occurrence Handle10817267
I.Y. Pieters E.A.P. Maeyer ParticleDe R.M.H. Verbeeck (1998) ArticleTitleInfluence of Na+ on the stoichiometry of carbonated hydroxyapatite obtained by the hydrolysis of octacalcium phosphate Inorg. Chem. 37 6392–6395 Occurrence Handle10.1021/ic9802810 Occurrence Handle1:CAS:528:DyaK1cXntVeru7c%3D
A.J. Salgado O.P. Coutinho R.L. Reis (2004) ArticleTitleBone tissue engineering: state of the art and future trends Macromol. Biosci. 4 743–765 Occurrence Handle10.1002/mabi.200400026 Occurrence Handle1:CAS:528:DC%2BD2cXntlCqsrY%3D Occurrence Handle15468269
E. Schwartz (1997) Culture of specific cell types, mesenchymal cells, bone R.I. Freshney (Eds) Culture of Animal Cells. A Manual of Basic Technique EditionNumber3 Wiley–Liss, Inc. New York 332–333
C.A. Scotchford M.G. Cascone S. Downes P. Giusti (1998) ArticleTitleOsteoblast responses to collagen–PVA bioartificial polymers in vitro: the effects of cross-linking method and collagen content Biomaterials 19 1–11 Occurrence Handle10.1016/S0142-9612(97)00236-6 Occurrence Handle1:STN:280:DyaK1czktF2rtg%3D%3D Occurrence Handle9678844
L.A. Solchaga P. Cassiède A.I. Caplan (1998) ArticleTitleDifferent response to osteo-inductive agents in bone-marrow and periosteum-derived cell preparations Acta Orthop. Scand. 69 IssueID4 426–432 Occurrence Handle1:STN:280:DyaK1M%2FhsFWqsg%3D%3D Occurrence Handle9798456
R.S. Spitzer C. Perka K. Lindenhayn H. Zippel (2002) ArticleTitleMatrix engineering for osteogenic differentiation of rabbit periosteal cells using α-tricalcium phosphate particles in a three-dimensional fibrin culture J. Biomed. Mater. Res. 59 690–696 Occurrence Handle10.1002/jbm.1277 Occurrence Handle1:CAS:528:DC%2BD38XksFCitw%3D%3D Occurrence Handle11774331
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Declercq, H.A., De Ridder, L.I. & Cornelissen, M.J. Isolation and Osteogenic Differentiation of Rat Periosteum-derived Cells. Cytotechnology 49, 39–50 (2005). https://doi.org/10.1007/s10616-005-5167-z
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10616-005-5167-z