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Cytotechnology

, Volume 47, Issue 1–3, pp 127–131 | Cite as

Relations Between Individual Cellular Motions and Proliferative Potentials in Successive Cultures of Human Keratinocytes

  • Norihiko Hata
  • Yuka Agatahama
  • Masahiro Kino-oka
  • Masahito Taya
Short Communication

Abstract

In the successive cultures of human keratinocyte cells, cellular motions of extension and rotation were analyzed based on observation of the individual cells, to evaluate the proliferative potential in a whole cell population. In lag phases of the serial cultures, an extension index of individual cells, R E , was defined as an average spreading rate divided by initial cell area for each cell. The mean value of R E was found to relate to prolongation of lag time; namely it decreased with increasing passage number in the successive cultures approaching cellular senescence. During the courses of the cultures, the rotation rate of paired cells was also measured through time-lapse observation. The mean value of rotation rate, \(| \bar{\omega}|\), decreased with an increase in doubling time caused by the progress of cellular age, reaching an almost constant value of \(| \bar{\omega}| =40\) h-1 in the cultures with prolonged doubling time of over 59 h. It was concluded that the indices determined from the motions of individual cells, R E and \(| \bar{\omega}|\), were correlated with the lag time and doubling time, respectively, which are growth parameters varied with the vitality of the cells approaching cellular senescence.

Keywords

Cell extension Cell rotation Doubling time Human keratinocytes Lag time Motion analysis 

Abbreviations

AC

projected area of each cell (μ m2)

AC0

initial projected area of each cell (μ m2)

n

number of examined cells (cells)

Nd

cumulative number of population doublings

Ndf

final value of cumulative number of population doublings

NP

number of passages

rs

spreading rate of each cell (μ m2 h-1)

rsave

average spreading rate of each cell (μ m2  h-1)

RE

cell extension index (h-1)

\(\bar {R} _{\rm E}\)

mean value of RE (h-1)

t

observation time (h)

td

doubling time (h)

tL

lag time (h)

\(|\omega|\)

absolute value of rotation rate (degree h-1)

\(|\bar {\omega}|\)

mean value of \(|\omega|\), (degree h-1)

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References

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Copyright information

© Springer 2005

Authors and Affiliations

  • Norihiko Hata
    • 1
  • Yuka Agatahama
    • 1
  • Masahiro Kino-oka
    • 1
  • Masahito Taya
    • 1
    • 2
  1. 1.Division of Chemical Engineering, Graduate School of Engineering ScienceOsaka UniversityToyonakaJapan
  2. 2.Graduate School of Frontier BiosciencesOsaka UniversityToyonakaJapan

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