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Primary Adipocyte Culture: Adipocyte Purification Methods May Lead to a New Understanding of Adipose Tissue Growth and Development

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Abstract

In the present manuscript, the methods required to generate purified cultures of mature adipocytes, as well as stromal vascular cells, from the same isolation are detailed. Also, we describe the in vitro conditions for the dedifferentiation of the isolated mature adipocytes. These two types of cells may be used to reevaluate differences between presently available cellular models for lipogenesis/lipolysis and might provide a new cellular physiological system for studies utilizing the proliferative progeny from mature adipocyte dedifferentiation. Alternative possibilities to the dedifferentiation phenomenon are proposed, as this new area of research is novel.

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Abbreviations

DMEM:

Dulbecco’s modified Eagle medium

DMEM/F12:

1:1 ratio; Dulbecco’s modified Eagle medium + Ham’s F12

FBS:

fetal bovine serum

HBSS:

Hank’s balanced salt solution

HS:

horse serum

PBS:

phosphate buffered saline, pH 7.08

PSG:

pigskin gelatin

SC:

satellite cell

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Authors and Affiliations

  1. Department of Animal Sciences, Washington State University, Pullman, WA, 99164, USA

    M. E. Fernyhough, J. L. Vierck & M. V. Dodson

  2. Richard B. Russell Research Center, USDA, ARS, Athens, GA, 30604, USA

    G. J. Hausman

  3. Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, T1J 4B1, Canada

    P. S. Mir

  4. Agricultural Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2E1, Canada

    E. K. Okine

Authors
  1. M. E. Fernyhough
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  2. J. L. Vierck
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  3. G. J. Hausman
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  4. P. S. Mir
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  5. E. K. Okine
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  6. M. V. Dodson
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Corresponding author

Correspondence to M. V. Dodson.

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Fernyhough, M.E., Vierck, J.L., Hausman, G.J. et al. Primary Adipocyte Culture: Adipocyte Purification Methods May Lead to a New Understanding of Adipose Tissue Growth and Development. Cytotechnology 46, 163–172 (2004). https://doi.org/10.1007/s10616-005-2602-0

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  • DOI: https://doi.org/10.1007/s10616-005-2602-0

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