Chemistry of Natural Compounds

, Volume 52, Issue 2, pp 296–298 | Cite as

Sterols and Fatty Acids Isolated from Laboratory Culture of the Toxic Marine Diatom Pseudo-nitzschia pungens

  • I. V. Stonik
  • I. I. Kapustina
  • N. A. Aizdaicher
  • V. I. Svetashev

Marine diatoms of the genus Pseudo-nitzschia H. Peragallo are widely distributed in the world’s oceans and are of interest because 16 of 44 known species of this genus contain amnesic toxin (domoic acid) [1, 2, 3] that accumulates in edible mollusks and fish through food chains and causes serious poisoning in people and marine fauna [4, 5, 6, 7]. The chemical compositions of many Pseudo-nitzschia are still little studied despite the interest in this group of diatoms. Their non-toxic metabolites are insufficiently studied [8]. The exceptions are fatty acid derivatives bacillariolides, which were isolated earlier from Pseudo-nitzschia pungens (Grunow ex Cleve) G. R. Hasle [9].

Herein, we communicate data on the isolation and structural analysis of sterol and fatty-acid fractions from algologically pure non-axenic culture of P. pungens (clone PPR-12). Developed methods were used to isolate the diatom in the culture [10]. The stock culture was inoculated into ten 1000-mL Ehrlenmeyer...


EtOAc Domoic Acid Marine Diatom Fatty Acid Derivative Dienol 
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The analysis of sterol and FA fractions was supported by RFBR Grant No. 15-04-05643a. The cultivation work was supported by the “Far East” Program No. 15-I-6-005o. We thank Dr. T. N. Makar′eva (PIBOC, FEB, RAS) for useful discussions.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • I. V. Stonik
    • 1
  • I. I. Kapustina
    • 2
  • N. A. Aizdaicher
    • 1
  • V. I. Svetashev
    • 1
  1. 1.A. V. Zhirmunsky Institute of Marine Biology, Far-East BranchRussian Academy of SciencesVladivostokRussia
  2. 2.G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-East BranchRussian Academy of SciencesVladivostokRussia

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