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Chemistry of Natural Compounds

, Volume 52, Issue 2, pp 296–298 | Cite as

Sterols and Fatty Acids Isolated from Laboratory Culture of the Toxic Marine Diatom Pseudo-nitzschia pungens

  • I. V. Stonik
  • I. I. Kapustina
  • N. A. Aizdaicher
  • V. I. Svetashev
Article

Marine diatoms of the genus Pseudo-nitzschia H. Peragallo are widely distributed in the world’s oceans and are of interest because 16 of 44 known species of this genus contain amnesic toxin (domoic acid) [1, 2, 3] that accumulates in edible mollusks and fish through food chains and causes serious poisoning in people and marine fauna [4, 5, 6, 7]. The chemical compositions of many Pseudo-nitzschia are still little studied despite the interest in this group of diatoms. Their non-toxic metabolites are insufficiently studied [8]. The exceptions are fatty acid derivatives bacillariolides, which were isolated earlier from Pseudo-nitzschia pungens (Grunow ex Cleve) G. R. Hasle [9].

Herein, we communicate data on the isolation and structural analysis of sterol and fatty-acid fractions from algologically pure non-axenic culture of P. pungens (clone PPR-12). Developed methods were used to isolate the diatom in the culture [10]. The stock culture was inoculated into ten 1000-mL Ehrlenmeyer...

Keywords

EtOAc Domoic Acid Marine Diatom Fatty Acid Derivative Dienol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgment

The analysis of sterol and FA fractions was supported by RFBR Grant No. 15-04-05643a. The cultivation work was supported by the “Far East” Program No. 15-I-6-005o. We thank Dr. T. N. Makar′eva (PIBOC, FEB, RAS) for useful discussions.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • I. V. Stonik
    • 1
  • I. I. Kapustina
    • 2
  • N. A. Aizdaicher
    • 1
  • V. I. Svetashev
    • 1
  1. 1.A. V. Zhirmunsky Institute of Marine Biology, Far-East BranchRussian Academy of SciencesVladivostokRussia
  2. 2.G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-East BranchRussian Academy of SciencesVladivostokRussia

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