Sterols and Fatty Acids Isolated from Laboratory Culture of the Toxic Marine Diatom Pseudo-nitzschia pungens
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Marine diatoms of the genus Pseudo-nitzschia H. Peragallo are widely distributed in the world’s oceans and are of interest because 16 of 44 known species of this genus contain amnesic toxin (domoic acid) [1, 2, 3] that accumulates in edible mollusks and fish through food chains and causes serious poisoning in people and marine fauna [4, 5, 6, 7]. The chemical compositions of many Pseudo-nitzschia are still little studied despite the interest in this group of diatoms. Their non-toxic metabolites are insufficiently studied . The exceptions are fatty acid derivatives bacillariolides, which were isolated earlier from Pseudo-nitzschia pungens (Grunow ex Cleve) G. R. Hasle .
Herein, we communicate data on the isolation and structural analysis of sterol and fatty-acid fractions from algologically pure non-axenic culture of P. pungens (clone PPR-12). Developed methods were used to isolate the diatom in the culture . The stock culture was inoculated into ten 1000-mL Ehrlenmeyer...
KeywordsEtOAc Domoic Acid Marine Diatom Fatty Acid Derivative Dienol
The analysis of sterol and FA fractions was supported by RFBR Grant No. 15-04-05643a. The cultivation work was supported by the “Far East” Program No. 15-I-6-005o. We thank Dr. T. N. Makar′eva (PIBOC, FEB, RAS) for useful discussions.
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