Polysaccharides from the Leaves of Lonicera japonica
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The polysaccharides from the leaves of Lonicera japonica (LLJPS) were extracted using Herbal Blitzkrieg Extractor (HBE) and separated into three fractions using 30%, 50%, and 80% ethanol by stepwise ethanol precipitation. Every fraction was prepared using a series of processes including deproteinization, decoloration, desalination, and lyophilization. The polysaccharide content was determinated by the phenol-sulfuric acid method .
The three fractions were further purified with DEAE-52 cellulose. The neutral polysaccharides, eluted with distilled water, were named LLJPS-30%N and LLJPS-80%N. The acidic polysaccharides, eluted respectively by the stepwise addition of NaCl solutions of different concentrations, were named LLJPS-50%A1, LLJPS-50%A2, and LLJPS-80%A.
The five fractions were isolated by Sephacryl S-300 and Sephadex G-100 column chromatography successively.
LLJPS-80%N and LLJPS-50%A2 gave a negative response to the Bradford test and no absorption at 280 and 260 nm in the UV...
KeywordsXylose Ferulic Acid Rhamnose Glucuronic Acid Galacturonic Acid
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