Chemistry of Natural Compounds

, Volume 52, Issue 2, pp 288–289 | Cite as

Polysaccharides from the Leaves of Lonicera japonica

  • Lei Liu
  • Qin Shang
  • Fugang Liu
  • Min Xu

The polysaccharides from the leaves of Lonicera japonica (LLJPS) were extracted using Herbal Blitzkrieg Extractor (HBE) and separated into three fractions using 30%, 50%, and 80% ethanol by stepwise ethanol precipitation. Every fraction was prepared using a series of processes including deproteinization, decoloration, desalination, and lyophilization. The polysaccharide content was determinated by the phenol-sulfuric acid method [1].

The three fractions were further purified with DEAE-52 cellulose. The neutral polysaccharides, eluted with distilled water, were named LLJPS-30%N and LLJPS-80%N. The acidic polysaccharides, eluted respectively by the stepwise addition of NaCl solutions of different concentrations, were named LLJPS-50%A1, LLJPS-50%A2, and LLJPS-80%A.

The five fractions were isolated by Sephacryl S-300 and Sephadex G-100 column chromatography successively.

LLJPS-80%N and LLJPS-50%A2 gave a negative response to the Bradford test and no absorption at 280 and 260 nm in the UV...


Xylose Ferulic Acid Rhamnose Glucuronic Acid Galacturonic Acid 
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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of PharmacyChildren′s Hospital of ZhengzhouZhengzhouP. R. China
  2. 2.Department of Pharmacy and DeviceHospital 273 of PLAKorlaP. R. China
  3. 3.College of PharmacyHenan University of Traditional Chinese MedicineZhengzhouP. R. China

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