Chemistry of Natural Compounds

, Volume 52, Issue 1, pp 74–77 | Cite as

Changes in Gastrodia tuber Ethanol Extracts During Grifola frondosa Fermentation

  • Xiao-bao Xu
  • Tian-xiang Wu
  • Qing-li Tang

Gastrodia tuber ethanol extracts can stimulate production of exopolysaccharide and biomass in Grifola frondosa. The main components of G. tuber ethanol extracts were assayed and their metabolic transformations by G. frondosa monitored by HPLC-UV-MS. Gastrodin, p-hydroxybenzyl alcohol, p-hydroxybenzaldehyde, and parishin were identified. The change in gastrodin residue (–268 U) was caused by parishin cracking (121°C, 0.15 MPa), decreasing the gastrodin residue content by 46.84% during 7 days fermentation. The findings will be of use in Chinese medicine processing technology.


ethanol extracts Gastrodia tuber gastrodin Grifola frondosa HPLC-UV-MS parishin 



This work was financially supported by the Natural Science Foundation of China (No. 31060272 in 2011) and Zhejiang University (No. 588040-172210217).


  1. 1.
    H. Nanba, J. Nat. Med., 1, 10 (1993).Google Scholar
  2. 2.
    H. Nanba, Explore (N.Y.), 6, 74 (1995).Google Scholar
  3. 3.
    Y. Adachi, N. Ohno, and T. Yadomae, Biol. Pharm. Bul., 21, 278 (1998).CrossRefGoogle Scholar
  4. 4.
    I. Suzuki, K. Hashimoto, S. Oikawa, K. Sato, M. Osawa, and T. Yadomae, Chem. Pharm. Bull., 37, 410 (1989).CrossRefPubMedGoogle Scholar
  5. 5.
    A. Inoue, N. Kodama, and H. Nanba, Biol. Pharm. Bul., 25, 536 (2002).CrossRefGoogle Scholar
  6. 6.
    B. C. Lee, J. T. Bae, H. B. Pyo, T. B. Choe, S. W. Kim, H. J. Hwang, and J. W. Yun, Enzyme. Microb. Tech., 32, 574 (2003).CrossRefGoogle Scholar
  7. 7.
    D. Gary, L. Hong, S. Andrew, F. Monica, D. A. Gabriella, W. Kathleen, Y. Simon, C. R. Susanna, J. V. Andrew, and C. Barrie, J. Cancer. Res. Clin. Oncol., 135, 1215 (2009).CrossRefGoogle Scholar
  8. 8.
    L. EnShyh, J. Microbiol. Biotechnol., 27, 555 (2011).CrossRefGoogle Scholar
  9. 9.
    C. L. Liu, M. C. Liu, and P. L. Zhu, Chromatographia, 55, 317 (2002).CrossRefGoogle Scholar
  10. 10.
    L. Wang, H. B. Xiao, X. M. Liang, and L. X. Wei, J. Sep. Sci., 30, 1488 (2007).CrossRefPubMedGoogle Scholar
  11. 11.
    J. H. Kim, J. T. Bae, M. H. Song, G. S. Lee, and H. B. Pyo, Grifola frondosa, Arch. Pharm. Res., 33, 1943 (2010).CrossRefPubMedGoogle Scholar
  12. 12.
    G. Q. Liu and K. Z. Zhang, Appl. Microbiol. Biot., 74, 572 (2007).CrossRefGoogle Scholar
  13. 13.
    G. Q. Liu, H. X. Xiao, X. L. Wang, Y. Zhao, Y. G. Zhang, and G. P. Ren, Appl. Biochem. Biotechnol., 165, 87 (2011).CrossRefPubMedGoogle Scholar
  14. 14.
    L. Wang, H. B. Xiao, L. Yang, and Z. T. Wang, J. Aslan. Nat. Prod. Res., 14, 457 (2012).CrossRefGoogle Scholar
  15. 15.
    J. Sarry and Z. Gunata, Food. Chem., 87, 509 (2004).CrossRefGoogle Scholar
  16. 16.
    P. Sunmin, S. K. Da, and K. Suna, Eur. J. Nutr., 50, 107 (2011).CrossRefGoogle Scholar
  17. 17.
    X. B. Xu, T. X. Wu, and F. Wang, Gastrodia Tuber, Afr. J. Biotechnol., 11, 3656 (2012).Google Scholar
  18. 18.
    N. I. Baek, S. Y. Choi, J. K. Park, S. W. Cho, E. M. Ahn, S. G. Jeon, B. R. Lee, J. H. Bahn, Y. K. Kim, and I. H. Shon, Arch. Pharm. Res., 22, 219 (1999).CrossRefPubMedGoogle Scholar
  19. 19.
    N. Wang, T. X. Wu, Y. Zhang, X. X. Xu, S. Tan, and H. W. Fu, Arch. Pharm. Res., 36, 314 (2013).CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.School of Chemical EngineeringGuizhou UniversityGuiyangP. R. China
  2. 2.School of Life SciencesGuizhou UniversityGuiyangP. R. China

Personalised recommendations