Chemistry of Natural Compounds

, Volume 51, Issue 3, pp 565–567 | Cite as

Catechins and Proanthocyanidins from Seeds of Fraxinus americana

  • Arafat Aybek
  • Junjie Zhou
  • Aybek Malik
  • Shahira Umar
  • Zhenghua Xiao

Perennial plants of the genus Fraxinus L. (Oleaceae) are broadly distributed in North America and Central and Eastern Asia. About 70 plant species in this genus, of which >20 species grow in China, were described [1]. We reported previously on the observation of new compounds in seeds of F. americana L. [2]. In continuation of research on this plant species growing in Xinjiang Autonomous Region, China, we isolated another eight phenolic compounds.

Air-dried seeds of F. americana (3.1 kg) were extracted (5 × 6 L) with aqueous Me2CO (60%) at room temperature. The obtained aqueous Me2CO extracts were evaporated in vacuo to 1 L. The condensed extract was diluted with H2O (1:1) and worked up sequentially with Et2O (4 × 500 mL), EtOAc (4 × 500 mL), and n-BuOH (4 × 500 mL). The solvents were evaporated to afford Et2O (30.6 g), EtOAc (180 g), and n-BuOH (120 g) fractions.

The Et 2O extract (30.6 g) was chromatographed over a column of silica gel (5 × 180 cm, 100/200 mesh, 800 g) with elution...


MeOH EtOAc Vanillin AcOH Et2O 
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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Arafat Aybek
    • 1
  • Junjie Zhou
    • 2
  • Aybek Malik
    • 3
  • Shahira Umar
    • 4
  • Zhenghua Xiao
    • 5
  1. 1.Copenhagen University, Department of Food Science, Faculty of Sciences DK-2702CopenhagenDenmark
  2. 2.Siegfried (Nantong) Pharmaceutical Co., Ltd. CASNantongP. R. China
  3. 3.Dongli (Nantong) Chemical Co., Ltd. CASNantongP. R. China
  4. 4.Xinjiang University of Chemistry and Chemical Engineering CASUrumqiP. R. China
  5. 5.Sichuan University of Science and Engineering of Chemical and Pharmaceutical Engineering CASZigongP. R. China

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