Catechins and Proanthocyanidins from Seeds of Fraxinus americana
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Perennial plants of the genus Fraxinus L. (Oleaceae) are broadly distributed in North America and Central and Eastern Asia. About 70 plant species in this genus, of which >20 species grow in China, were described . We reported previously on the observation of new compounds in seeds of F. americana L. . In continuation of research on this plant species growing in Xinjiang Autonomous Region, China, we isolated another eight phenolic compounds.
Air-dried seeds of F. americana (3.1 kg) were extracted (5 × 6 L) with aqueous Me2CO (60%) at room temperature. The obtained aqueous Me2CO extracts were evaporated in vacuo to 1 L. The condensed extract was diluted with H2O (1:1) and worked up sequentially with Et2O (4 × 500 mL), EtOAc (4 × 500 mL), and n-BuOH (4 × 500 mL). The solvents were evaporated to afford Et2O (30.6 g), EtOAc (180 g), and n-BuOH (120 g) fractions.