Dehydroepiandrosterone (1) was incubated with a cell suspension culture of Codiaeum variegatum for biotransformational studies. After 10 days of incubation, four metabolites, 5α-androstane-3,17-dione (2), 5β-androstane-3,17-dione (3), androst-4-ene-3,17-dione (4), and 17β-hydroxyandrost-4-en-3-one (5), were obtained. The structures of these biotransformed products were elucidated by spectroscopic methods. Biotransformation using a cell suspension culture of Codiaeum variegatum provides an effective and convenient method to oxidize the 3β-hydroxy group in 1.
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One of us (Azizuddin) acknowledges the enabling role of the Federal Urdu University of Arts, Science & Technology, Gulshan-e-Iqbal, Karachi, Pakistan, and appreciate its financial support through the “Mini Research Project.”