Liquid Chromatography Coupled with Q-TOF Mass Spectrometry for the Characterization of Phenolics from Acacia Catechu
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Liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) has been recognized as a powerful analytical tool for the identification of phytochemicals due to its high sensitivity, short run time, and less use of toxic organic solvents used as mobile phase compared to reversed-phase standalone HPLC coupled with an diode-array detector . Accurate mass measurement is an important feature of Q-TOF MS/MS, which is useful to differentiate compounds with the same nominal mass but dissimilar exact masses. Generation of free radicals or reactive oxygen species (ROS) during metabolism and other activities beyond the antioxidant capacity of a biological system gives rise to oxidative stress , which plays a role in heart diseases, neurodegenerative diseases, cancer, and in the aging process . This concept is supported by increasing evidence indicating that oxidative damage plays a role in the development of chronic, age-related degenerative diseases, and that dietary...
KeywordsQuercetin Match Percentage Phenol Carboxylic Acid Photo Diode Array Detector Base Peak Chromatogram
The authors are thankful to the authorities of Arya Vaidya Sala Kottakkal and the Vice Chancellor of Karpagam University for extending the facilities and TATA Trust, Mumbai for financial assistance.
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