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Deglycosylation of isoflavonoid glycosides from Maackia amurensis cell culture by β−D-glucosidase from Littorina sitkana hepatopancrease

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Chemistry of Natural Compounds Aims and scope

Maakia amurensis (strain A-18) cell culture synthesizes a significant quantity of isoflavonoids, a large part of which consists of isoflavone glucosides and malonylglucosides. β−D-Hydrolase enzyme complexes from the marine mollusk Littorina sitkana and the marine mycelial fungus P. canescens were used to obtain isoflavones from their conjugated forms. The specificity of β−D-glucanases from L. sitkana for various glycosides was studied. The deglycosylation efficiency depended on the aglycon structure. The deglycosylated fraction of isoflavonoids obtained from M. amurensis cell culture exhibited antitumor activity.

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Acknowledgment

The work was supported by RFBR grant 09-04-00761, RAS Presidium Program Molecular and Cellular Biology, and Presidential Grant MK 1472.2009.4.

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Authors and Affiliations

  1. Pacific Institute of Bioorganic Chemistry, Far-East Branch, Russian Academy of Sciences, 690022, Vladivostok, Russia

    M. I. Kusaikin, A. M. Zakharenko, S. P. Ermakova, M. V. Veselova, E. V. Grigoruk, S. A. Fedoreev & T. N. Zvyagintseva

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  1. M. I. Kusaikin
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  2. A. M. Zakharenko
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  3. S. P. Ermakova
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  4. M. V. Veselova
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  5. E. V. Grigoruk
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  6. S. A. Fedoreev
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  7. T. N. Zvyagintseva
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Corresponding author

Correspondence to M. I. Kusaikin.

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Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 182–185, March– April, 2011.

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Kusaikin, M.I., Zakharenko, A.M., Ermakova, S.P. et al. Deglycosylation of isoflavonoid glycosides from Maackia amurensis cell culture by β−D-glucosidase from Littorina sitkana hepatopancrease. Chem Nat Compd 47, 197–200 (2011). https://doi.org/10.1007/s10600-011-9880-x

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  • DOI: https://doi.org/10.1007/s10600-011-9880-x

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