Chemistry of Natural Compounds

, Volume 46, Issue 6, pp 1008–1009 | Cite as

Identification of the amino-acid composition of cellulolytic enzymes by a phenylthiocarbamoyl method

  • Sh. Ya. Mirzaakhmedov
  • D. T. Ruzmetova
  • N. Sh. Berdiev
  • Sh. I. Salikhov

The fungus Aspergillus terreus is known to produce highly active cellulolytic and xylanolytic enzymes depending on the cultivation conditions [1, 2, 3]. The enzymes can be used for biodegradation of ligno-cellulose wastes [4] and to destroy plant cell walls [5]. Knowledge of the physicochemical properties of the enzymes is needed in order to apply them successfully in various economic sectors.

Our goal was to study the physicochemical properties of cellulase-II, xylanase-I, xylanase-II, and pectinase isolated from A. terreus by determining the amino-acid composition, the N-terminus amino acids, and the isoelectric points (pI).

The aforementioned enzymes were isolated and purified by the literature methods [1, 6]. The classical method for determining the amino-acid composition of proteins and peptides consist of separating their acid hydrolysates using ionexchange chromatography with subsequent identification as ninhydrin derivatives [7]. We used a highly sensitive phenylthiocarbamoyl...


Isoelectric Point CH3CN Literature Method CH3COONa Methanesulfonic Acid 
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Copyright information

© Springer Science+Business Media, Inc. 2011

Authors and Affiliations

  • Sh. Ya. Mirzaakhmedov
    • 1
  • D. T. Ruzmetova
    • 1
  • N. Sh. Berdiev
    • 1
  • Sh. I. Salikhov
    • 1
  1. 1.A. S. Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of UzbekistanTashkentUzbekistan

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