Identification of the amino-acid composition of cellulolytic enzymes by a phenylthiocarbamoyl method
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The fungus Aspergillus terreus is known to produce highly active cellulolytic and xylanolytic enzymes depending on the cultivation conditions [1, 2, 3]. The enzymes can be used for biodegradation of ligno-cellulose wastes  and to destroy plant cell walls . Knowledge of the physicochemical properties of the enzymes is needed in order to apply them successfully in various economic sectors.
Our goal was to study the physicochemical properties of cellulase-II, xylanase-I, xylanase-II, and pectinase isolated from A. terreus by determining the amino-acid composition, the N-terminus amino acids, and the isoelectric points (pI).
The aforementioned enzymes were isolated and purified by the literature methods [1, 6]. The classical method for determining the amino-acid composition of proteins and peptides consist of separating their acid hydrolysates using ionexchange chromatography with subsequent identification as ninhydrin derivatives . We used a highly sensitive phenylthiocarbamoyl...
KeywordsIsoelectric Point CH3CN Literature Method CH3COONa Methanesulfonic Acid
- 1.Sh. Ya. Mirzaakhmedov, Zh. F. Ziyavitdinov, Z. R. Akhmedova, A. B. Saliev, D. T. Ruzmetova, Kh. B. Ashurov, D. Fessas, and S. Iametti, Khim. Prir. Soedin., 489 (2007).Google Scholar
- 6.Sh. Ya. Mirzaakhmedov, D. T. Ruzmetova, and N. Sh. Berdiev, in: Proceedings of the International Scientific Conference “Critical Problems in Bioorganic Chemistry” [in Russian], Tashkent, Sept. 20-21, 2010, pp. 24, 71.Google Scholar