Chemistry of Natural Compounds

, Volume 46, Issue 6, pp 837–840 | Cite as

Structure of carbohydrate antigens from Microbulbifer sp. KMM 6242

  • R. P. Gorshkova
  • V. V. Isakov
  • O. I. Nedashkovskaya
  • E. L. Nazarenko

A capsular polysaccharide (CPS) containing D-galactosamine uronic acid and D-alanine was isolated from a culture of the marine proteobacterium Microbulbifer sp. KMM 6242. 2D NMR spectroscopy showed that the CPS is a homopolymer of 2-acetamido-2-deoxy-N-(D-galacturonyl)-D-alanine with the structure →4)-β-D-GalpNAcA6(D-Ala)-(1→. An O-specific polysaccharide containing D-ribose and D-galactose was isolated from the cell-membrane lipopolysaccharide. 1D and 2D NMR spectroscopy established the structure of the disaccharide repeating unit of the polysaccharide as →3)-β-D-Ribf-(1→4)-β-D-Galp-(1→.


Microbulbifer capsular polysaccharide O-specific polysaccharide NMR spectroscopy 



The work was partially supported by grants of the RFBR 08-04-12069-ofi, the FEB RAS No. 09-Sh-A-05-142, and the RF President NSh-2383-2008.4.


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Copyright information

© Springer Science+Business Media, Inc. 2011

Authors and Affiliations

  • R. P. Gorshkova
    • 1
  • V. V. Isakov
    • 1
  • O. I. Nedashkovskaya
    • 1
  • E. L. Nazarenko
    • 1
  1. 1.Pacific Institute of Bioorganic Chemistry, Far-East BranchRussian Academy of SciencesVladivostokRussia

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