Chemistry of Natural Compounds

, Volume 45, Issue 1, pp 32–39 | Cite as

Structure of oligomeric proanthocyanidines from Hedysarum thienum roots studied by thiolysis and MALDI-TOF MS

  • I. V. Nechepurenko
  • N. I. Komarova
  • Yu. V. Gerasimova
  • V. V. Koval′
  • M. P. Polovinka
  • D. V. Korchagina
  • N. F. Salakhutdinov

Oligomeric proanthocyanidines from Hedysarum theinum roots that were cleaved by benzylmercaptan were shown to be heterogeneous mixtures consisting of prodelphinine and procyanidine structural units with the former dominating. Fractionation of oligomeric proanthocyanidines on polyamide and MCI gel CHP20P sorbent isolated fractions containing primarily mixtures of di- and trimeric, tri- and tetrameric, or tetra- and pentameric proanthocyanidines. Analysis by mass spectrometry (MALDI-TOF) showed that fractions of trimers and tetramers contained more proanthocyanidines with a single A-type bond than fractions of dimers and trimers.

Key words

Hedysarum theinum Krasnob. Fabaceae oligomeric proanthocyanidines A-type proanthocyanidines condensed tannins acid cleavage thiolysis mass spectrometry MALDI-TOF 


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Copyright information

© Springer Science+Business Media, Inc. 2009

Authors and Affiliations

  • I. V. Nechepurenko
    • 1
  • N. I. Komarova
    • 1
  • Yu. V. Gerasimova
    • 2
    • 3
  • V. V. Koval′
    • 2
    • 3
  • M. P. Polovinka
    • 1
    • 3
  • D. V. Korchagina
    • 1
  • N. F. Salakhutdinov
    • 1
  1. 1.N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian BranchRussian Academy of SciencesNovosibirskRussia
  2. 2.Institute of Chemical Biology and Fundamental Medicine, Siberian BranchRussian Academy of SciencesNovosibirskRussia
  3. 3.Novosibirsk State UniversityNovosibirskRussia

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