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Study of immobilized and extracellular invertase of lemon balm

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Chemistry of Natural Compounds Aims and scope

Cell suspensions of lemon balm (Melissa officinalis L.) were permeabilized by Tween 20, Tween 80, ethanol, hexadecyltrimethylammonium bromide, and hexadecylpyridinium chloride, and immobilized by glutaraldehyde. The invertase pH optimum was 4.5 at temperature 50°C. The hydrolysis of substrate was linear for 4 h, reaching 60% conversion. The cells had high invertase activity and good stability, and in longterm storage they showed good physicomechanical properties. The culture medium (without cells) was used for the identification and determination of extracellular enzyme activity. Intracellular activity was estimated from the cell suspension. For the lemon balm cell suspension, the intracellular activity accounted for 83.7% of the total activity, and the extracellular one for 12.7%. The intracellular specific activity is 4.2 times higher. Our method permits the rapid, simple, and specific identification and determination of plant invertase.

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Acknowledgment

This work was partially supported by the Grant Agency VEGA (Bratislava) grant No 1/3289/06. We thank Mrs. Anna Hutyrova and Mr. Peter Keckes for technical assistance.

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Authors and Affiliations

  1. Garden of Medicinal Plants, Faculty of Pharmacy, Comenius University, Odbojarov 10, SK-832 32, Bratislava, Slovakia

    J. Stano & M. Korenova

  2. Department of Botany, Faculty of Natural Sciences, Comenius University, Revova 39, SK-812 02, Bratislava, Slovakia

    K. Micieta

  3. Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Odbojarov 10, SK-832 32, Bratislava, Slovakia

    V. Blanarikova

  4. Institute of Biochemistry, Martin Luther University, K. Mothes Strasse 3, D-061 20, Halle, Germany

    H. Tintemann

  5. 900 32 Borinka pri Bratislave, Podhradska, 380, Slovakia

    P. Nemec

  6. Research Institute of Vegetables, Andovska 6, SK-940 01, Nove Zamky, Slovakia

    M. Valsikova

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  1. J. Stano
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Correspondence to K. Micieta.

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Published in Khimiya Prirodnykh Soedinenii, No. 6, pp. 611-615, November-December, 2008.

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Stano, J., Micieta, K., Korenova, M. et al. Study of immobilized and extracellular invertase of lemon balm. Chem Nat Compd 44, 755–761 (2008). https://doi.org/10.1007/s10600-009-9183-7

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  • DOI: https://doi.org/10.1007/s10600-009-9183-7

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