Isolation and purification of lignoperoxidase from the mushroom Pleurotus ostreatus

  • Sh. Ya. Mirzaakhmedov
  • Zh. F. Ziyavitdinov
  • Z. R. Akhmedova
  • A. B. Saliev
  • D. T. Ruzmetova
  • S. T. Azizov
  • Dimitrios Fessas
  • Stefania Iametti
Article

Abstract

The accumulation dynamics of lignolytic enzymes in culture media of the basidiomycetes Panus tigrinus, Pleurotus ostreatus, Fomes fomentarus, and the micromycete Aspergillus terreus were studied during the incubation period. It was found that Pleurotus ostreatus is the most active producer of lignoperoxidase enzymes among the studied fungi. Gel filtration and ion-exchange chromatography were used to isolate a homogeneous enzyme with lignoperoxidase activity. The maximum activity was found at pH 2.7 and 29°C. Gel electrophoresis determined the molecular weight (44 kDa).

Key words

ligninase Pleurotus ostreatus lignoperoxidase activity chromatography electrophoresis 

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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • Sh. Ya. Mirzaakhmedov
    • 1
  • Zh. F. Ziyavitdinov
    • 1
  • Z. R. Akhmedova
    • 2
  • A. B. Saliev
    • 1
  • D. T. Ruzmetova
    • 1
  • S. T. Azizov
    • 3
  • Dimitrios Fessas
    • 4
  • Stefania Iametti
    • 4
  1. 1.Institute of Bioorganic ChemistryTashkentUzbekistan
  2. 2.Institute of MicrobiologyTashkentUzbekistan
  3. 3.Institute of ElectronicsTashkentUzbekistan
  4. 4.University of Milan, DISTAMMilanoItaly

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