Comparison on the antioxidant capacity of selected fruits and vegetables and their separations
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Several kinds of fruits, including apple, pear, and grape, and vegetables, including onion and tomato, were studied in this paper. FRAP and DPPH assays were used to determine the antioxidant activity of ethanol extracts and their separations. After being extracted, different phenolic compounds were separated on Sephadex LH-20 column. EC1 and EC50 were used to show the ferric reducing ability and DPPH scavenging activity. The results indicated that the Fe-reducing ability order of different extracts was different with that of DPPH scavenging ability. The mutual effect of the substances in the mixture influenced the antioxidant activity of the separations.
Key wordspolyphenols separations antioxidant capacity FRAP DPPH
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