Chemistry of Natural Compounds

, Volume 41, Issue 5, pp 588–591 | Cite as

HPLC-MS Screening of the Antioxidant Profile of Italian Olive Cultivars

  • G. Sivakumar
  • C. Briccoli Bati
  • N. Uccella


Freeze-dried olive fruits from Italian cultivars such as Carolea, Cassanese, and Coratina were analyzed by HPLC-MS. During different stages of maturation of olive fruits, the biophenolic (antioxidant) composition was examined. Twelve biophenolic compounds, viz. hydroxytyrosol, tyrosol, oleoside 11-methyl ester, demethyloleuropein, verbascoside, demethylligstoside, oleacin, oleuropeindiale, demethyloleuropeindiale, oleuropein, ligstroside, and elenolate were identified by HPLC-MS. Oleuropein, the major olive fruit soluble biophenolic fraction, decreased significantly during fruit maturation and showed significant differences between olive cultivars. Coratina drupes extracted from October month contained a higher amount of biophenols than other mono-cultivar extracted drupes.

Key words

antioxidant beta glucosidase demethyloleuropein oleuropein olive 


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  1. 1.
    N. Uccella and A. Saija, Trends food Sci. & Technol., 11, 357 (2001).Google Scholar
  2. 2.
    N. Uccella, G. Bisignano, and A. Tomaino, J. Pharm. Pharmacol., 51, 971 (1999).PubMedGoogle Scholar
  3. 3.
    N. Uccella, Trends Food Sci. & Technol., 11, 328 (2001).Google Scholar
  4. 4.
    F. Gutierrez-Rosales, J. J. Rios, and Ma. L. Gomez-Rey, J. Agric. Food Chem., 51, 6021 (2003).PubMedCrossRefGoogle Scholar
  5. 5.
    N. Uccella, A. Bianco, C. Melchioni, A. Ramunno, and G. Romeo, Nat. Prod. Res., 18, 29 (2004).PubMedGoogle Scholar
  6. 6.
    A. Bianco and N. Uccella, Food Res. Int., 33, 475 (2000).CrossRefGoogle Scholar
  7. 7.
    N. Uccella, A. Bianco, F. Buiarelli, G. Cartoni, F. Coccioli, I. Muzzalupo, and A. Polidori, Anal. Lett., 34, 1033 (2001).Google Scholar
  8. 8.
    D. Ryan, M. Antolovich, T. Herlt, P. D. Prenzler, S. Lavee, and K. Robards, J. Agric. Food Chem., 50, 6716 (2002).PubMedCrossRefGoogle Scholar
  9. 9.
    R. Briante, M. Patumi, S. Limongelli, F. Febbraio, C. Vaccaro, A. D. Salle, F. L. Cara, and R. Nucci, Plant Science, 162, 791 (2002).CrossRefGoogle Scholar
  10. 10.
    J. R. Morello, M. P. Romero, T. Ramo, and M. J. Motilva, Plant Sci., 168, 65 (2005).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • G. Sivakumar
    • 1
  • C. Briccoli Bati
    • 2
  • N. Uccella
    • 3
  1. 1.ENEA, Biotech GenomicsCasaccia, RomeItaly
  2. 2.Istituto Sperimentale per la Olive coltura, C. Da Li RocchiRende (CS)Italy
  3. 3.CIRASAIACalabria UniversityArcavacata, Rende (CS)Italy

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