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Conservation Genetics

, Volume 12, Issue 5, pp 1333–1344 | Cite as

Genetic variation in Delonix s.l. (Leguminosae) in Madagascar revealed by AFLPs: fragmentation, conservation status and taxonomy

  • Malin C. Rivers
  • Neil A. Brummitt
  • Eimear Nic Lughadha
  • Thomas R. Meagher
Research Article

Abstract

The distribution of genetic diversity has potential to inform conservation efforts but is rarely incorporated when conservation status is assigned to a species. These data can be beneficial to the conservation assessment process by providing information on subpopulations, gene flow and effective population sizes, thus achieving more successful assessments. In order to obtain a better understanding of the patterns of genetic variation and their relationship to conservation in the fragmented flora of Madagascar, this study assessed genetic diversity among and within Delonix s.l. (Leguminosae) using AFLP markers. The genetic diversity of eight species of Delonix s.l. (covering 79 sample sites and 254 individuals) showed a range of values (25–61% for polymorphic loci, and 0.076–0.192 Shannon’s Index). Results from an analysis of molecular variance (AMOVA) suggest that a majority of the genetic variance is attributed to variation within species (87%), which is also supported by a principle coordinate analysis of genetic distances between sites. The results were used to compare the genetic difference between species of different threat status and show that even closely related species with the same IUCN threat status differ in their genetic structure, probably arising from differences in life history traits, pollen and seed dispersal, and fragmentation. Species that are recently affected by habitat destruction and fragmentation are likely to be at high potential risk of genetic erosion contributing to their ongoing decline. Thus, genetic variation should be taken into consideration in conservation assessments, whenever possible, to provide accurate and targeted conservation recommendations in order to achieve more successful conservation outcomes.

Keywords

AFLP Conservation status Delonix Fragmentation Madagascar Taxonomy 

Notes

Acknowledgments

We thank ANGAP and the Ministère pour la Production Animale et des Eaux et Forêts for their permission to work in Madagascar. We are grateful for the support in Madagascar by the Kew Madagascar Office and PBZT, in particular H. Razafindraibe and J. Razanatsoa for their invaluable support in the field. Special thanks also to D. Forbes at University of St Andrews for help with the lab work and to R.J. Smith, J. Graves and R. Abbott for comments on an earlier version of the manuscript. This work was supported by the Natural Environment Research Council NER/S/A/2006/14303 and the Leverhulme Trust (TRM).

Supplementary material

10592_2011_234_MOESM1_ESM.doc (82 kb)
Online Resource 1 (a)–(d) Pairwise genetic distances (ΦPT) among (a) Delonix decaryi, (b) Delonix floribunda, (c) Delonix pumila and (d) Delonix velutina. Significant comparisons (P < 0.05) are marked with * based on random permutations (N = 9,999). (Significant comparisons (P < 0.05) after sequential Bonferroni test for multiple comparisons are seen in bold). Label numbers refers to sample site. (DOC 82 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Malin C. Rivers
    • 1
    • 2
  • Neil A. Brummitt
    • 3
  • Eimear Nic Lughadha
    • 1
  • Thomas R. Meagher
    • 2
  1. 1.Royal Botanic GardensKew, RichmondUK
  2. 2.Centre for Evolution, Genes & Genomics, School of BiologyUniversity of St AndrewsSt AndrewsUK
  3. 3.Department of BotanyNatural History MuseumLondonUK

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