Characterization of ten polymorphic microsatellite markers for the protected Spanish moon moth Graellsia isabelae (Lepidoptera: Saturniidae)
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Ten polymorphic microsatellite loci were developed for Graellsia isabelae. Polymorphism was assessed for 20 individuals from a Spanish population (Els-Ports-de-Beseit, Catalonia) and 39 more individuals from one population in the French Alps and six other Spanish localities. Overall, the number of alleles per locus ranged from 5 to 24. Els-Ports-de-Beseit showed an average number of alleles per locus of 9.80 (SD = 4.32), observed heterozygosity was 0.71 (SD = 0.226), and expected heterozygosity was 0.788 (SD = 0.146). Genotypic frequencies conformed to Hardy–Weinberg equilibrium at the Catalonian population, and no evidence for linkage disequilibrium was observed. Multilocus genotypes resulting from this set of markers will be useful to determine genetic diversity and differentiation within and among populations of this highly protected moth. Several loci amplified and resulted polymorphic in two related species: two loci in Actias neidhoeferi, and three loci in A. luna.
KeywordsGraellsia isabelae Actias Microsatellites Conservation genetics Lepidoptera Saturniidae
John Ciseski provided us with samples of Actias luna. Nuria Remon kindly helped us in the lab. We thank J. Casaponsa, A. Cervelló, Y.-L. Chen, F. Goussard, S. Ibáñez, R. Maciá, J. Martí, MICROFAUNA, T. Latasa, J. Nevado, A. Sáez, L.-C. Shih, W.-C. Tsao, and J. Ylla for their help during fieldwork. David Lees improved an earlier version of the manuscript with his comments. We appreciate the support provided by Ecogenics GmbH, Parc Natural dels Ports, and Departament de Medi Ambient (Generalitat de Catalunya). This work was performed under permits from all environmental authorities concerned. It was financially supported by the Spanish Ministry of Education and Science (CGL2007-63549/BOS). NMM was funded by a FPI grant (BES-2008-002571). MV was funded by Xunta de Galicia (Isidro Parga Pondal programme) and European Science Foundation (CONGEN short-visit (1635) and exchange (1683) grants). Sampling of Actias neidhoeferi was supported by National Science Council (97-2621-B-110-001-MY3).
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