SNP discovery based on CATS and genotyping in the finless porpoise (Neophocaena phocaenoides)
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Single nucleotide polymorphisms (SNPs) discovery and genotyping were performed for the finless porpoises (Neophocaena phocaenoides). About 202 comparative anchor tagged sequence primers derived from genomes of human, mouse and some other mammals were used to screen the finless porpoise population. Of the 51 SNPs discovered, 25 were further characterized with ideal genotyping primers and using fragment length discrepant allele specific PCR assay. This is the first report of SNP loci for the finless porpoise, which is helpful to provide some novel molecular markers and new genetic information relevant to the conservation and management of this endangered species.
KeywordsNeophocaena phocaenoides SNPs CATS FLDAS-PCR
This research was supported by the National Natural Science Foundation of China (NSFC) key project grant no. 30830016 and NSFC project grant no. 30770294, the Program for New Century Excellent Talents in University (NCET-07-0445), the Ministry of Education of China, the Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP 20060319002), the Ministry of Education of China, and the major project of the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (grant no. 07KJA18016) awarded to Dr. Yang. The authors thank Anli Gao, Xin-Rong Xu, Hua Chen, and Qing Chang for collecting sample.
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