Advertisement

Conservation Genetics

, 10:1989 | Cite as

Characterization of polymorphic microsatellites for the rough periwinkle gastropod, Littorina saxatilis (Olivi, 1792) and their cross-amplification in four congeners

  • Caitríona E. McInerney
  • A. Louise Allcock
  • Mark P. Johnson
  • Paulo A. Prodöhl
Technical Note

Abstract

Eight new microsatellite loci were characterized for Littorina saxatilis (Olivi, 1792) and tested for their cross-hybridization in congeners. All loci were polymorphic in Irish and Celtic Sea samples, with an average number of alleles per locus of 15 (range, 6–31). Observed and expected locus heterozygosities ranged from 26 to 85% and from 53 to 92%, respectively. Three loci showed excess homozygosity and significant departures from Hardy–Weinberg expectations in one sample, possibly due to null alleles, population structuring or inbreeding. No linkage disequilibrium was detected among loci within samples. A high degree of cross-hybridization was observed in closely related congeners and most loci were polymorphic. These markers will be useful for investigating population genetic diversity and connectivity in coastal populations, especially for marine reserve design.

Keywords

Littorina saxatilis Enrichment Microsatellite Gastropod Population genetics Littorina 

Notes

Acknowledgments

C. McInerney was supported by a grant from the Higher Education Authority, Ireland awarded to M. P. Johnson, A. L. Allcock, and P. A. Prodöhl. We thank J. Nunn, O. Mulholland, M. Jessopp, J. Leal-Flórez and M. Hughes for assistance with fieldwork and helpful discussions.

References

  1. Bell JB (2008) Similarity in connectivity patterns for two gastropod species lacking pelagic larvae. Mar Ecol Prog Ser 357:185–194. doi: 10.3354/meps07301 CrossRefGoogle Scholar
  2. Chapuis MP, Estoup A (2007) Microsatellite null alleles and estimation of population differentiation. Mol Biol Evol 24:621–631. doi: 10.1093/molbev/msl191 CrossRefPubMedGoogle Scholar
  3. Conde-Padín P, Cruz R, Hollander J et al (2008) Revealing the mechanisms of sexual isolation in a case of sympatric and parallel ecological divergence. Biol J Linn Soc Lond 94:513–526. doi: 10.1111/j.1095-8312.2008.00998.x CrossRefGoogle Scholar
  4. Falush D, Stephens M, Pritchard JK (2007) Inference of population structure using multilocus genotype data: dominant markers and null alleles. Mol Ecol Notes 7:574–578. doi: 10.1111/j.1471-8286.2007.01758.x CrossRefPubMedGoogle Scholar
  5. Guillot G, Santos F, Estoup A (2008) Analysing georeferenced population genetics data with Geneland: a new algorithm to deal with null alleles and a friendly graphical user interface. Bioinformatics 24:1406–1407. doi: 10.1093/bioinformatics/btn136 CrossRefPubMedGoogle Scholar
  6. Johannesson K (2003) Evolution in Littorina: ecology matters. J Sea Res 49:107–117. doi: 10.1016/S1385-1101(02)00218-6 CrossRefGoogle Scholar
  7. Kijas JMH, Fowler JCS, Gabett CA et al (1994) Enrichment of microsatellites from the citrus genome using biotinylated oligonucleotide sequences bound to streptavidin-coated magnetic particles. Biotechniques 16:657–662Google Scholar
  8. McInerney CE, Allcock AL, Johnson MP et al (2009) Characterization of polymorphic microsatellites for the periwinkle gastropod, Littorina littorea (Linnaeus, 1758) and their cross-amplification in four congeners. Conserv Genet (in press)Google Scholar
  9. Panova M, Mäkinen T, Fokin M et al (2008) Microsatellite cross-species amplification in the genus Littorina and detection of null alleles in Littorina saxatilis. J Molluscan Stud 74:111–117. doi: 10.1093/mollus/eym052 CrossRefGoogle Scholar
  10. Prodöhl PA, Loughry WJ, McDonough CM (1996) Molecular documentation of polyembryony and the micro-spatial dispersion of clonal sibships in the nine-banded armadillo, Dasypus novemcinctus. Proc R Soc Lond B Biol Sci 263:1643–1649. doi: 10.1098/rspb.1996.0240 CrossRefGoogle Scholar
  11. Raymond M, Rousset F (1995) GENEPOP (Version 3.4): population genetic software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
  12. Reid DG (1996) Systematics and evolution of Littorina. The Ray Society, LondonGoogle Scholar
  13. Sokolov EP, Sokolova IM, Pörtner HO (2002) Polymorphic microsatellite DNA markers from the marine gastropod Littorina saxatilis. Mol Ecol Notes 2:27–29. doi: 10.1046/j.1471-8286.2002.00133.x CrossRefGoogle Scholar
  14. Taggart JB, Hynes RA, Prodöhl PA et al (1992) A simplified protocol for routine total DNA isolation from salmonid fishes. J Fish Biol 40:963–965. doi: 10.1111/j.1095-8649.1992.tb02641.x CrossRefGoogle Scholar
  15. van Oosterhout C, Hutchinson WF, Wills DPM et al (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538. doi: 10.1111/j.1471-8286.2004.00684.x CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Caitríona E. McInerney
    • 1
  • A. Louise Allcock
    • 1
    • 2
  • Mark P. Johnson
    • 2
  • Paulo A. Prodöhl
    • 1
  1. 1.School of Biological Sciences, Medical Biology CentreQueen’s University BelfastBelfastNorthern Ireland
  2. 2.Martin Ryan Marine Science InstituteNational University of Ireland GalwayGalwayIreland

Personalised recommendations