Conservation Genetics

, 10:1957 | Cite as

A simple method for isolation of microsatellites from the mudskipper (Boleophthalmus pectinirostris), without constructing a genomic library

  • Shou-Jie Tang
  • Zhi-Zhi Liu
  • Wen-Qiao Tang
  • Jin-Quan Yang
Technical Note


The mudskipper Boleophthalmus pectinirostris is an important aquaculture species in China. It is endangered due to the loss and fragmentation of natural habitat, seawater pollution and overfishing. In this study, ten polymorphic microsatellite loci were isolated by the 5′-anchored polymerase chain reaction technique. Population genetic parameters were estimated on 60 wild individuals collected from Jiuduansha wetland in Yangtze River estuary. The number of alleles ranged from 3 to 14. The observed(H O) and expected(H E) heterozygosities ranged from 0.2500 to 0.8500 and from 0.3679 to 0.8692, respectively. This suite of microsatellite loci will facilitate future studies of the genetic status of wild and hatchery bred populations of B. pectinirostris.


Boleophthalmus pectinirostris Microsatellite 5′-anchored PCR 



We are grateful to Ms. Sang-Ni Lu, Mr. Ding He, Ms. Qi Ding, Mr. Qing-Hui Meng and Mr. Wei Feng for the help in lab work. This research was supported by the grants from Shanghai Leading Academic Discipline Project (S30701, Y1101).


  1. Akihito SK, Ikeda Y, Iwata A (2000) Suborder gobioidei. In: Nakabo T (ed) Fishes of Japan with pictorial keys to the species. Tokai University Press, Tokyo, pp 1139–1628 (in Japanese)Google Scholar
  2. Brachet S, Jubier MF, Richard M, Jung-Muller B, Frascaria-Lacoste N (1999) Rapid identification of microsatellite loci using 5′-anchored PCR in the common ash Fraxinus excelsior. Mol Ecol 8:157–168Google Scholar
  3. Excoffier L, Laval G, Schneider S (2005) ARLEQUIN (version 3.0): an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50PubMedGoogle Scholar
  4. Fisher PJ, Gardner RC, Richardson TE (1996) Single locus microsatellites isolated using 5′-anchored PCR. Nucleic Acids Res 24:4369–4371. doi: 10.1093/nar/24.21.4369 CrossRefPubMedGoogle Scholar
  5. Jin CH, Zhong AH, Huang FY, Zhang CD, Li MY (2004) The research on genetic diversity of Boleophthalmus pectinirostris L. from natural population by RAPD method. Mar Sci 12:26–30Google Scholar
  6. Kanemori Y, Takegaki T, Natsukari Y (2006) Genetic population structure of Boleophthalmus pectinirostris inferred from mitochondrial DNA sequences. Jpn J Ichthyol 53:133–141Google Scholar
  7. Liu ZZ, Yang JQ, Wang ZQ, Tang WQ (2009) Genetic structure and population history of Boleophthalmus pectinirostris in Yangtze River Estuary and its southern adjacent regions. Zool Res 1:1–10CrossRefGoogle Scholar
  8. Murdy EO (1989) A taxonomic revision and cladistic analysis of the oxudercine gobies (Gobiaidae: Oxudercinae). Rec Aust Mus Suppl 11:1–93Google Scholar
  9. O’Connell M, Wright M (1997) Microsatellite DNA in fishes. Rev Fish Biol Fish 7:331–363. doi: 10.1023/A:1018443912945 CrossRefGoogle Scholar
  10. Provan J, Wilson PJ (2007) Development of microsatellites for the peat moss Sphagnum capillifolium using ISSR cloning. Mol Ecol Notes 7:254–256. doi: 10.1111/j.1471-8286.2006.01572.x CrossRefGoogle Scholar
  11. Rice WR (1989) Analyzing tables of statistical tests. Evol Int J Org Evol 43:223–225. doi: 10.2307/2409177 Google Scholar
  12. Rozen S, Skaletsky HJ (2000) Primer 3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386Google Scholar
  13. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
  14. Zhang QY, Hong WS (2006) Review and prospect of mudskipper Boleophthalmus pectinirostris studies. J Xiamen Univ (Natural Science) Sup 2:97–108Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Shou-Jie Tang
    • 1
  • Zhi-Zhi Liu
    • 1
  • Wen-Qiao Tang
    • 1
  • Jin-Quan Yang
    • 1
  1. 1.Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of EducationShanghai Ocean UniversityShanghaiChina

Personalised recommendations