Twenty-three polymorphic microsatellite markers for the Caribbean endemic Zenaida dove, Zenaida aurita, and its conservation in related Zenaida species
Twenty-three polymorphic microsatellite loci, six dinucleotidic loci and 17 tetranucleotidic loci, were developed for the Zenaida dove (Zenaida aurita), a bird species endemic to the Caribbean Islands. From a set of 30 individuals captured at one single location in Barbados, we obtained 20 loci that did not deviate from Hardy–Weinberg Equilibrium. Number of alleles per locus ranged between 2 and 11 (average 7.05) and the expected heterozygosity per locus, He ranged between 0.321 and 0.881 (average 0.712). This gives an exclusionary power for parental analysis of 0.9999 and 1.0000, knowing the genotype of one social parent, or both, respectively. Such results indicate that these 20 loci will be useful for both studying population genetics and mate choice patterns in Z. aurita. All 20 loci amplified in four other Zenaida species, the Galápagos dove, Z. galapagoensis, the eared dove, Z. auriculata, the mourning dove, Z. macroura, the Pacific dove, Z. meloda, with 30–96% being polymorphic.
KeywordsEndemic species Extra-pair paternity Gene flow Mate choice Microsatellite Zenaida dove
This work was supported by grants to F. C. and R. W. by “Institut Buffon” Université de Bourgogne, to K. M. from the French Ministère de l’Education Nationale (grant # 01121) and to D. S.-A. and P. G. P. from the Whitney R. Harris World Ecology Centre, Idea Wild, The Frank M. Chapman Memorial Fund of the American Museum of Natural History, The Field Research for Conservation program of the Saint Louis Zoo (FRC 05-2 and FRC 08-2), the Organization for Tropical Studies, and by the Des Lee collaborative vision in zoological studies. Logistical support and permits were provided by the Galápagos National Park, the Darwin Research Foundation and the Nature Heritage Department of Barbados. Authors thank Kenneth Jones and Mark Todd from Genetic Identification Services for their help and patience in developing the microsatellite loci, and Jérôme Moreau for helping collecting biological material.
- Barré N, Feldmann P, Tayalay G et al (1997) Introduction et extension de la tourterelle turque Streptopelia decaocto dans les Petites-Antilles. Alauda 65:245–250Google Scholar
- Goudet J (1995) FSTAT (vers. 1.2): a computer program to calculate F-statistics. J Hered 86:485–486Google Scholar
- Rivera-Milan FF (1997) Seasonal and annual changes in the population density of Zenaida doves in the xenophytic forest of Guanica, Puerto Rico. J Field Ornithol 68:259–272Google Scholar
- Sambrook J, Tritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NYGoogle Scholar
- Stutchbury BJM, Morton ES (2001) Behavioural ecology of tropical birds. Academic Press, New YorkGoogle Scholar
- Wattier R, Engel CR, Saumitou-Laprade P et al (1998) Short allele dominance as a source of heterozygote deficiency at microsatellite loci: experimental evidence at the dinucleotide locus Gv1CT in Gracilaria gracilis (Rhodophyta). Mol Ecol 7:1569–1573. doi: 10.1046/j.1365-294x.1998.00477.x CrossRefGoogle Scholar
- Wiley JW (1991) Ecology and behaviour of the Zenaida dove. Ornitol Neotrop 2:49–75Google Scholar