Conservation Genetics

, 10:1545 | Cite as

Characterization of twenty-six polymorphic microsatellite markers for the fox squirrel (Sciurus niger) and their utility in gray squirrels (Sciurus carolinensis) and red squirrels (Tamiasciurus hudsonicus)

  • Jennifer A. Fike
  • Olin E. RhodesJr
Technical Note


Twenty-six polymorphic microsatellite markers were developed for the fox squirrel (Sciurus niger). The number of alleles ranged from 2 to 11 and observed heterozygosities ranged from 0.037 to 0.815. Preliminary screening revealed that numerous loci also were polymorphic in other squirrel species: Sciurus carolinensis and Tamiasciurus hudsonicus. These loci should be useful tools for investigating the social structure, mating tactics and movement behavior of fox squirrels.


Fox squirrel Microsatellite Sciuridae Sciurus niger 



We thank Rob Swihart for assistance with obtaining fox squirrel tissues. We are grateful to our colleagues in the FNR genetics lab for their assistance and advice. Funding for this research was provided by the Wildlife Diversity Section of the Indiana Department of Natural Resources and Purdue University.


  1. Beheler AS, Fike JA, Murfitt LM, Rhodes OE Jr, Serfass TS (2004) Development of polymorphic microsatellite loci for North American river otters (Lontra canadensis) and amplification in related Mustelids. Mol Ecol Notes 4:56–58. doi: 10.1046/j.1471-8286.2003.00564.x CrossRefGoogle Scholar
  2. Hall ER (1981) The mammals of North America, 2nd edn. Wiley, New YorkGoogle Scholar
  3. Hamilton MB, Pincus EL, Di Fiore A, Fleischer RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. Biotechniques 27:500–507PubMedGoogle Scholar
  4. Hauswaldt SJ, Glenn TC (2003) Microsatellite DNA loci from the diamondback terrapin (Malaclemys terrapin). Mol Ecol Notes 3:174–176. doi: 10.1046/j.1471-8286.2003.00388.x CrossRefGoogle Scholar
  5. Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7:639–655. doi: 10.1046/j.1365-294x.1998.00374.x CrossRefPubMedGoogle Scholar
  6. Raymond M, Rousset F (2000) genepop version 3.4. Available at Accessed 20 Oct 2008
  7. Rozen S, Skaletsky HJ (2000) primer3. Available at Accessed 31 Aug 2007

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Department of Forestry and Natural ResourcesPurdue UniversityWest LafayetteUSA

Personalised recommendations