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Conservation Genetics

, 10:1469 | Cite as

Isolation and characterization of 10 polymorphic microsatellite loci from small yellow croaker (Pseudosciaena polyactis)

  • Song-Lin Chen
  • Shi-Chao Xing
  • Gen-Bo Xu
  • Xiaolin Liao
  • Jing-Feng Yang
Technical Note

Abstract

Small yellow croaker (Pseudosciaena polyactis) is an economically important marine fish species. About 43 microsatellite loci were isolated from two enriched genomic library of Pseudosciaena polyactis. Ten of these loci were polymorphic in a test population with alleles per locus ranging from two to six, and observed and expected heterozygosities per locus from 0.3750 to 0.8750 and from 0.3112 to 0.8121, respectively. No loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction and no significant linkage disequilibrium was found between pairs of loci. These polymorphic microsatellite loci would be useful for investigating genetic diversity of Pseudosciaena polyactis.

Keywords

Small yellow croaker Pseudosciaena polyactis Enriched genomic library SSR Microsatellites 

Notes

Acknowledgments

This study was supported by State 863 High-technology R&D project of China (2006AA10A403), Taishan Scholar Project of Shandong Province, National Public Platform of Science & Technology Information Resources (2005DKA30470-06).

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Song-Lin Chen
    • 1
  • Shi-Chao Xing
    • 1
    • 2
  • Gen-Bo Xu
    • 1
  • Xiaolin Liao
    • 1
  • Jing-Feng Yang
    • 1
  1. 1.Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdaoChina
  2. 2.College of Marine Life SciencesOcean University of ChinaQingdaoChina

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