Isolation and characterization of 12 microsatellite loci from cutlassfish (Trichiurus haumela)
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The cutlassfish (Trichiurus haumela) is an important commercial fish species. In the present study, we report 12 polymorphic microsatellite DNA markers for cutlassfish. The number of alleles per locus ranged from 2 to 9 in a sample of 26 individuals. Observed and expected heterozygosities per locus varied from 0.2727 to 0.9583 and from 0.4059 to 0.7926, respectively. Two loci (Trha25 and Trha27) showed significant departure from Hardy–Weinberg equilibrium after sequential Bonferroni correction (P < 0.0042). No significant linkage disequilibrium between pairs of loci was found. These microsatellite markers provide powerful tools for investigating genetic population structure, population history and conservation management of cutlassfish.
KeywordsCutlassfish Trichiurus haumela Microsatellites Genetic diversity
This study was supported by Taishan Scholar Project of Shandong Province, National Public Platform of Science & Technology Information Resources (2005DKA30470-06) and scientific foundation of YSFRI, CAFS (2007-qn-13).
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