Characterization of expressed sequence tag (EST)-derived microsatellite loci in the fire ant Solenopsis invicta (Hymenoptera: Formicidae)
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The fire ant Solenopsis invicta has received considerable attention as a damaging invasive pest. In the present study, 12 polymorphic microsatellite loci were developed from publicly available expressed sequence tags (ESTs) of S. invicta. The number of alleles varied between 3 and 12, with an average of 6.42 per locus. The observed and expected heterozygosities ranged from 0.4167 to 1.0000 and from 0.3446 to 0.8543, respectively. These informative EST-derived microsatellite loci provide a powerful complementary tool for genetic studies of S. invicta.
KeywordsFire ants Solenopsis invicta Expressed sequence tags EST-SSRs Microsatellites
This study was supported by grants to RHC from the Australian Research Council (DP0665890, DP0450569) and by the Endeavour Postgraduate Award to ZQQ from the Department of Education, Employment and Workplace Relations, Australia. BCS and FMS were supported by the Austrian Science Fund (J2639-B17, J2642-B17). Genotyping in this study was performed by the Genetic Analysis Facility at James Cook University. Non-RIFA voucher specimens (AEBZ-20 & AEJE) have been deposited in the Queensland Museum.
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