Isolation and characterisation of microsatellite loci in Calystegia soldanella (Convolvulaceae), an endangered coastal plant isolated in Lake Biwa, Japan
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Eight microsatellite loci of Calystegia soldanella useful for comparisons of the genetic structure of isolated populations in the ancient Lake Biwa and coastal populations in Japan were isolated and characterised. The number of alleles ranged from 2 to 5. The expected (H E) and observed (H O) heterozygosities were 0.097–0.583 and 0.000–0.380, respectively, from 100 individuals from Lake Biwa and coastal populations. Seven of the eight loci exhibited significantly fewer heterozygotes than expected based on the Hardy–Weinberg equilibrium (P < 0.05). These primers amplifying microsatellites in C. soldanella may provide a population genetics tool useful in the establishment of a conservation strategy.
KeywordsCalystegia soldanella Conservation genetics Heterozygosity Lake Biwa Microsatellite
We are grateful to Mutsumi Ogawa for cooperation in collecting samples. This study was supported by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan and the Japan Society for the Promotion of Science (19570085).
- Doyle JJ, Doyle JL (1990) Isolation of plant DNA from plant tissue. Focus 12:13–15Google Scholar
- Goudet J (1995) Fstat version 1.2: a computer program to calculate F statistics. J Hered 86:485–486Google Scholar
- Kitamura S (1968) Phytogeography of Shiga Prefecture. In: Kitamura S (ed) Flora of Shiga Prefecture. Hoikusha, Osaka, pp 25–33 (in Japanese)Google Scholar
- Rozen S, Skaletsky H (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386Google Scholar