Conservation Genetics

, Volume 10, Issue 4, pp 1057–1059 | Cite as

Development of microsatellite markers for the endangered grassland perennial herb Vincetoxicum atratum (Apocynaceae–Asclepiadoideae)

  • Fumito Tada
  • Tadashi Yamashiro
  • Masayuki Maki
Technical Note


Eight microsatellite markers were developed for the endangered grassland perennial herb Vincetoxicum atratum. The number of alleles ranged from 4 to 14, and the expected heterozygosities were from 0.575 to 0.933 in a population of V. atratum. Five of the eight loci did not significantly deviated from the Hardy–Weinberg equilibrium. All eight loci were tested for cross-species amplification in five other species of Vincetoxicum in Japan. These microsatellite loci will be useful for conservation genetics of V. atratum and other species of Vincetoxicum.


Apocynaceae Conservation genetics Cross-species amplification Microsatellite Vincetoxicum atratum 



This study was partly supported by a grant-in-aid from Japan Society for the Promotion of Science (JSPS). We are grateful to Messrs Y. Takahashi, T. Yamada, M. Takehara, and S. Iijima for sampling materials and Dr. S. Horie for technical advice and statistical analyses.


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  1. 1.Division of Ecology and Evolutionary Biology, Graduate School of Life SciencesTohoku UniversityAoba, SendaiJapan
  2. 2.Department of Life Science, Faculty of Integrated Arts and SciencesThe University of TokushimaTokushimaJapan

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