Development of microsatellite markers for the endangered grassland perennial herb Vincetoxicum atratum (Apocynaceae–Asclepiadoideae)
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Eight microsatellite markers were developed for the endangered grassland perennial herb Vincetoxicum atratum. The number of alleles ranged from 4 to 14, and the expected heterozygosities were from 0.575 to 0.933 in a population of V. atratum. Five of the eight loci did not significantly deviated from the Hardy–Weinberg equilibrium. All eight loci were tested for cross-species amplification in five other species of Vincetoxicum in Japan. These microsatellite loci will be useful for conservation genetics of V. atratum and other species of Vincetoxicum.
KeywordsApocynaceae Conservation genetics Cross-species amplification Microsatellite Vincetoxicum atratum
This study was partly supported by a grant-in-aid from Japan Society for the Promotion of Science (JSPS). We are grateful to Messrs Y. Takahashi, T. Yamada, M. Takehara, and S. Iijima for sampling materials and Dr. S. Horie for technical advice and statistical analyses.
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