Skip to main content

Advertisement

Log in

Development of microsatellite markers for the endangered grassland perennial herb Vincetoxicum atratum (Apocynaceae–Asclepiadoideae)

  • Technical Note
  • Published:
Conservation Genetics Aims and scope Submit manuscript

Abstract

Eight microsatellite markers were developed for the endangered grassland perennial herb Vincetoxicum atratum. The number of alleles ranged from 4 to 14, and the expected heterozygosities were from 0.575 to 0.933 in a population of V. atratum. Five of the eight loci did not significantly deviated from the Hardy–Weinberg equilibrium. All eight loci were tested for cross-species amplification in five other species of Vincetoxicum in Japan. These microsatellite loci will be useful for conservation genetics of V. atratum and other species of Vincetoxicum.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Bloor PA, Barker FS, Watts PC, Noyes HA, Kemp SJ (2001) Microsatellite libraries by enrichment. http://www.liv.ac.uk/~kempsj/genomics.html

  • Environment Agency of Japan (2007) The red list of vascular plants of Japan. http://www.biodic.go.jp/rdb/rdb_f.html (in Japanese)

  • Fischer D, Bachmann K (1998) Microsatellite enrichment in organisms with large genomes (Allium cepa L.). Biotechnique 24:796–802

    CAS  Google Scholar 

  • Maki M, Horie S (1999) Random amplified polymorphic DNA (RAPD) markers reveal less genetic variation in the endangered plant Cerastium fisherianum var. molle than in the widespread conspecific C. fisherianum var. fisherianum. Mol Ecol 8:145–150. doi:10.1046/j.1365-294X.1999.00517.x

    Article  CAS  Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (version 3.3): population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Rice W (1989) Analyzing tables of statistical tests. Evolution 43:223–225. doi:10.2307/2409177

    Article  Google Scholar 

  • Yamazaki T (1993) Asclepiadaceae. In: Iwatsuki K, Yamazaki T, Boufford DE, Ohba H (eds) Floral of Japan, vol IIIa. Kodansha, Tokyo, pp 168–182

Download references

Acknowledgements

This study was partly supported by a grant-in-aid from Japan Society for the Promotion of Science (JSPS). We are grateful to Messrs Y. Takahashi, T. Yamada, M. Takehara, and S. Iijima for sampling materials and Dr. S. Horie for technical advice and statistical analyses.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Masayuki Maki.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tada, F., Yamashiro, T. & Maki, M. Development of microsatellite markers for the endangered grassland perennial herb Vincetoxicum atratum (Apocynaceae–Asclepiadoideae). Conserv Genet 10, 1057–1059 (2009). https://doi.org/10.1007/s10592-008-9687-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10592-008-9687-x

Keywords

Navigation