Abstract
The silky sifaka, Propithecus candidus, considered one of the rarest and most endangered primates in the world, exists in only a few fragmented forests in northeastern Madagascar. This species faces increasing pressures as a direct result from loss of habitat in the form of tavy (slash and burn agriculture), illegal logging and mining along with hunting for subsistence, even within protected areas. We report a marker suite of 21 loci developed from genomic DNA from a silky sifaka collected in 2003 from Anjanaharibe-Sud Special Reserve. Polymorphism of each locus evaluated in 18 individuals pooled from Marojejy National Park and Anjanaharibe-Sud Special Reserve. The number of observed alleles per locus ranges from 2 to 7. The observed and expected heterozygosity were 0.389–0.889 and 0.322–0.789, respectively. The information revealed in this study will provide useful tools for further study of the social structure and population dynamics of the silky sifaka to facilitate conservation management in the imminent future.
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References
Dieringer D, Schlötterer C (2003) Microsatellite analyzer (MSA): a platform independent analysis tool for large microsatellite data sets. Mol Ecol Notes 3:167–169. doi:10.1046/j.1471-8286.2003.00351.x
Goudet J (1995) FSTAT, a computer program to test F-statistics. J Hered 86:85–486
Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available: http://www.unil.ch/izea/softwares/fstat.html. Updated from Goudet (1995)
Hillis DM, Mable BK, Larson A et al (1996) Nucleic acids IV: sequencing and cloning. In: Hillis DM, Moritz C, Zimmer EA (eds) Molecular systematics, 2nd edn. Sinauer Associates, Inc, Sunderland, MA, pp 321–381
Kandpal RP, Kandpal G, Weissman SM (1994) Construction of libraries enriched for sequence repeats and jumping clones, and hybridization selection for region-species markers. Proc Natl Acad Sci USA 91:88–92. doi:10.1073/pnas.91.1.88
Longmire JL, Gee GF, Hardekoff CL, Mark GA (1982) Establishing paternity in whooping cranes (Grus americana) by DNA analysis. Auk 109:522–529
Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7(5):639–655. doi:10.1046/j.1365-294x.1998.00374.x
Mittermeier RA, Konstant WR, Hawkins F et al (2006) Lemurs of Madagascar, 2nd edn. Conservation International Tropical Field Guide Series, Washington, DC, pp 383–387
Mittermeier RA, Ratsimbazafy J, Rylands AB et al (2007) Primates in Peril: The world’s 25 most endangered primates, 2006–2008. Primate Conserv 22:1–40
Moraga-Amador D, Farmerie BA, Brazeau D, Clark G (2001) “Tools for developing molecular markers.” Interdisciplinary center for biotechnology research laboratory manual. The University of Florida Gainesville, Florida, pp 11–65
Patel E (2006) Activity budget, ranging and group size in silky sifakas (Propithecus candidus). Lemur News 11:42–45
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecuminicisms. J Hered 86:248–249
Sambrook J, Fritch EF, Maniatus T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Press, New York
Slate J, Marshall TC, Pemberton JM (2000) A retrospective assessment of the accuracy of the paternity inference program CERVUS. Mol Ecol 9(6):801–808. doi:10.1046/j.1365-294x.2000.00930.x
Van Oosterhaut C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538. doi:10.1111/j.1471-8286.2004.00684.x
Acknowledgements
The authors wish to recognize the Malagasy field guides; this project would not have been possible without them. Thanks to the staff from the Institute for Conservation of Tropical Environments, Madagascar (ICTE-MICET), the Association Nationale pour la Gestion des Aires Protégées (ANGAP) and the Ministère des Eaux et Foret, Madagascar. This research was supported through a kindhearted grant from the Ahmanson Foundation and the generosity of Bill and Berniece Grewcock. We would also like to extend our thanks to the Hubbard Family Foundation for their belief in and ongoing contributions to the Madagascar Biodiversity & Biogeography Project.
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McGuire, S.M., Emodi, G.P., Shore, G.D. et al. Characterization of 21 microsatellite marker loci in the silky sifaka (Propithecus candidus). Conserv Genet 10, 985–988 (2009). https://doi.org/10.1007/s10592-008-9668-0
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DOI: https://doi.org/10.1007/s10592-008-9668-0