Characterisation of microsatellite loci in the habitat-forming kelp, Ecklonia radiata (Phaeophyceae, Laminariales)
Habitat forming algae play an important role in the ecology of temperate reefs worldwide. Despite this, our understanding of levels of gene flow within and among populations of algae is largely limited to studies on intertidal species; we know comparatively little about important habitat-forming subtidal algae. Here, we develop eight polymorphic microsatellite markers for the characterisation of population genetic diversity and structure in the subtidal kelp, Ecklonia radiata. This large macroalga is the most abundant habitat-forming kelp on the subtidal rocky reefs of temperate Australia and New Zealand where it forms extensive forests that support an astounding diversity of associated taxa.
KeywordsEcklonia radiata Microsatellites Kelp Habitat forming
We thank Drs. Brendan Kelaher and Nathan Knott and Ms. Louise McKenzie for help with fieldwork, Matthew Hayden for assisting with the STM technique and Dr. Joe Valentine for E. radiata samples from Tasmania. This work was funded by an Australian Research Council Postdoctoral Fellowship to M.A. Coleman.
- Belkhir K, Borsa P, Chikhi L, Raufaste N, Bonhomme F (2000) Genetix, a windows™ based software for population genetic analyses. Laboratoire Génome, Populations, Interactions CNRS UMR 5000, Université de Montpellier II, Montpellier, France. http://www.univ-montp2.fr/~genetix/genetix.htm
- Goudet J (1995) FSTAT (ver. 1.2): a computer program to calculate F-statistics. J Hered 86:485–486Google Scholar
- Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, NJ, pp 365–386Google Scholar