Fifteen novel polymorphic microsatellites in rock carp, Procypris rabaudi (Tchang), an endemic fish species in the upper reaches of the Yangtze River drainage
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Fifteen new microsatellite markers were isolated in rock carp, Procypris rabaudi (Tchang), from an AC-enriched genomic library. The allele number of these loci ranged from three to 13 (average 6.87 per locus) in the tested 24 individuals. Polymorphism information content (PIC) ranged from 0.453 to 0.821 with an average of 0.719. Average observed and expected heterozygosities were 0.712 (range 0.565–0.917) and 0.772 (range 0.542–0.855), respectively. These microsatellite markers could be useful in the population genetic study of rock carp.
KeywordsRock carp Procypris rabaudi Microsatellite Polymorphism
This work was funded by the National Natural Science Foundation of China (no. 30670290) and 973 Project (No. 2007CB411605). We are grateful to Prof. Xuefu He, and Mr. Chunsun Zhang for their help in sample collecting.
- Bloor PA, Barker FS, Watts PC, Noyes HA, Kemp SJ (2001) Microsatellite libraries by enrichment. URL: http://www.liv.ac.uk/-kempsj/genomics.html. Accessed May 2004
- Ding RH (1994) The fishes of Sichuan, China. Sichuan Publishing House of Science and Technology, Chengdu, Sichuan, ChinaGoogle Scholar
- Duan XB, Chen DQ, Liu SP, Chi CG, Yang RH (2002) Studies on status of fishery resources in three gorges reservoir reaches of the Yangtze River. Acta Hydrobiol Sin 26(6):605–611Google Scholar
- Hartl DL, Clark AG (1997) Principle of population genetics. Sinauer Associates, Sunderland, MAGoogle Scholar
- Raymond M, Rousset F (2002) GENEPOP version 3.3 (online). Available at http://wbiomed.curtin.edu.au/genepop
- Rozen S, Skaletsky HJ (2000) Primer 3.0. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, New Jersey, pp 365–386Google Scholar
- Sambrook T, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New YorkGoogle Scholar
- Wang S (1998) China Red Data Book of endangered animals: Pisces. Science Press, Beijing, ChinaGoogle Scholar