Fifteen novel polymorphic microsatellites in rock carp, Procypris rabaudi (Tchang), an endemic fish species in the upper reaches of the Yangtze River drainage
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Fifteen new microsatellite markers were isolated in rock carp, Procypris rabaudi (Tchang), from an AC-enriched genomic library. The allele number of these loci ranged from three to 13 (average 6.87 per locus) in the tested 24 individuals. Polymorphism information content (PIC) ranged from 0.453 to 0.821 with an average of 0.719. Average observed and expected heterozygosities were 0.712 (range 0.565–0.917) and 0.772 (range 0.542–0.855), respectively. These microsatellite markers could be useful in the population genetic study of rock carp.
KeywordsRock carp Procypris rabaudi Microsatellite Polymorphism
This work was funded by the National Natural Science Foundation of China (no. 30670290) and 973 Project (No. 2007CB411605). We are grateful to Prof. Xuefu He, and Mr. Chunsun Zhang for their help in sample collecting.
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